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Western blot
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ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [12]
- Comments [0]
- Validations
- Immunocytochemistry [2]
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- Product number
- ADI-SPA-400-F - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ADI-SPA-400-F, RRID:AB_11180881
- Product name
- HSP40/Hdj1 polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full length protein
- Description
- Protein A affinity purified.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Guinea Pig, Hamster, Porcine, Rabbit, Sheep, Simian, Xenopus
- Host
- Rabbit
- Vial size
- 200 μg
- Storage
- -20°C
- Handling
- Avoid freeze/thaw cycles.
Submitted references Reversibility of neuropathology and motor deficits in an inducible mouse model for FXTAS.
Huntingtin fragments and SOD1 mutants form soluble oligomers in the cell.
Association of simian virus 40 vp1 with 70-kilodalton heat shock proteins and viral tumor antigens.
Chemical and biological approaches synergize to ameliorate protein-folding diseases.
Neuronal expression of constitutive heat shock proteins: implications for neurodegenerative diseases.
Detection of novel intracellular alpha-synuclein oligomeric species by fluorescence lifetime imaging.
Hsp70 and Hsp40 improve neurite outgrowth and suppress intracytoplasmic aggregate formation in cultured neuronal cells expressing mutant SOD1.
Molecular chaperones enhance the degradation of expanded polyglutamine repeat androgen receptor in a cellular model of spinal and bulbar muscular atrophy.
Parkin accumulation in aggresomes due to proteasome impairment.
Geldanamycin activates a heat shock response and inhibits huntingtin aggregation in a cell culture model of Huntington's disease.
Polyglutamine length-dependent interaction of Hsp40 and Hsp70 family chaperones with truncated N-terminal huntingtin: their role in suppression of aggregation and cellular toxicity.
The Hdj-2/Hsc70 chaperone pair facilitates early steps in CFTR biogenesis.
Hukema RK, Buijsen RA, Schonewille M, Raske C, Severijnen LA, Nieuwenhuizen-Bakker I, Verhagen RF, van Dessel L, Maas A, Charlet-Berguerand N, De Zeeuw CI, Hagerman PJ, Berman RF, Willemsen R
Human molecular genetics 2015 Sep 1;24(17):4948-57
Human molecular genetics 2015 Sep 1;24(17):4948-57
Huntingtin fragments and SOD1 mutants form soluble oligomers in the cell.
Park YN, Zhao X, Norton M, Taylor JP, Eisenberg E, Greene LE
PloS one 2012;7(6):e40329
PloS one 2012;7(6):e40329
Association of simian virus 40 vp1 with 70-kilodalton heat shock proteins and viral tumor antigens.
Li PP, Itoh N, Watanabe M, Shi Y, Liu P, Yang HJ, Kasamatsu H
Journal of virology 2009 Jan;83(1):37-46
Journal of virology 2009 Jan;83(1):37-46
Chemical and biological approaches synergize to ameliorate protein-folding diseases.
Mu TW, Ong DS, Wang YJ, Balch WE, Yates JR 3rd, Segatori L, Kelly JW
Cell 2008 Sep 5;134(5):769-81
Cell 2008 Sep 5;134(5):769-81
Neuronal expression of constitutive heat shock proteins: implications for neurodegenerative diseases.
Chen S, Brown IR
Cell stress & chaperones 2007 Spring;12(1):51-8
Cell stress & chaperones 2007 Spring;12(1):51-8
Detection of novel intracellular alpha-synuclein oligomeric species by fluorescence lifetime imaging.
Klucken J, Outeiro TF, Nguyen P, McLean PJ, Hyman BT
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2006 Oct;20(12):2050-7
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2006 Oct;20(12):2050-7
Hsp70 and Hsp40 improve neurite outgrowth and suppress intracytoplasmic aggregate formation in cultured neuronal cells expressing mutant SOD1.
Takeuchi H, Kobayashi Y, Yoshihara T, Niwa J, Doyu M, Ohtsuka K, Sobue G
Brain research 2002 Sep 13;949(1-2):11-22
Brain research 2002 Sep 13;949(1-2):11-22
Molecular chaperones enhance the degradation of expanded polyglutamine repeat androgen receptor in a cellular model of spinal and bulbar muscular atrophy.
Bailey CK, Andriola IF, Kampinga HH, Merry DE
Human molecular genetics 2002 Mar 1;11(5):515-23
Human molecular genetics 2002 Mar 1;11(5):515-23
Parkin accumulation in aggresomes due to proteasome impairment.
Junn E, Lee SS, Suhr UT, Mouradian MM
The Journal of biological chemistry 2002 Dec 6;277(49):47870-7
The Journal of biological chemistry 2002 Dec 6;277(49):47870-7
Geldanamycin activates a heat shock response and inhibits huntingtin aggregation in a cell culture model of Huntington's disease.
Sittler A, Lurz R, Lueder G, Priller J, Lehrach H, Hayer-Hartl MK, Hartl FU, Wanker EE
Human molecular genetics 2001 Jun 1;10(12):1307-15
Human molecular genetics 2001 Jun 1;10(12):1307-15
Polyglutamine length-dependent interaction of Hsp40 and Hsp70 family chaperones with truncated N-terminal huntingtin: their role in suppression of aggregation and cellular toxicity.
Jana NR, Tanaka M, Wang Gh, Nukina N
Human molecular genetics 2000 Aug 12;9(13):2009-18
Human molecular genetics 2000 Aug 12;9(13):2009-18
The Hdj-2/Hsc70 chaperone pair facilitates early steps in CFTR biogenesis.
Meacham GC, Lu Z, King S, Sorscher E, Tousson A, Cyr DM
The EMBO journal 1999 Mar 15;18(6):1492-505
The EMBO journal 1999 Mar 15;18(6):1492-505
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Supportive validation
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Western Blot analysis of HSP40/Hdj1, pAb (Prod. No. SPA-400): Lane 1: M W Marker, Lane 2: HSP40/Hdj1 (human), (recombinant) Protein (Prod. No. SPP-400), Lane 3: HeLa (heat shocked), (cell lysate) (Prod. No. LYC-HL101) , Lane 4: 3T3 (heat shocked), (cell lysate) (Prod. No. LYC-3T101), Lane 5: PC-12, (cell lysate) (Prod. No. LYC-PC100), Lane 6: DnaJ (E. coli), (recombinant) (His-tag) Protein (Prod. No. SPP-640)(Negative Control)
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of human placenta tissue stained with HSP40/Hdj1, pAb at 10µg/ml.
