LS-C114331

antibody from LSBio

Targeting: POU5F1 MGC22487, OCT3, Oct4, OTF3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C114331

Provider

LSBio

Product name

POU5F1 / OCT4 Antibody (clone OTI9B7) LS-C114331

Antibody type

Monoclonal

Description

Protein A/G purified

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

OTI9B7

Storage

Store at -20°C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OCT-4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OCT-4.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Oct4 antibody (9B7) at 1:5000 dilution + lysates from HEK-293T cell transfected with human OCT4 expression vector.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC of paraffin-embedded Human endometrium tissue using anti-OCT4 mouse monoclonal antibody.