NBP2-15053

antibody from Novus Biologicals

Targeting: POU5F1 MGC22487, OCT3, Oct4, OTF3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-15053

Provider

Novus Biologicals

Product name

Rabbit Polyclonal OCT4 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: OCT4 Antibody [NBP2-15053] - Sample (20 ug of whole cell lysate) A: mouse ESC 12% SDS PAGE; antibody diluted at 1:10000.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: OCT4 Antibody [NBP2-15053] - Sample: cell lysate/extract of OCT4 gene transfected 293T cells A. Cell lysate/extract of transfected 293T cell, B. Control with 2 ug of preimmune rabbit IgG, C. Immunoprecipitation of OCT4 by 2 ug of OCT4 antibody 12% SDS-PAGE gel.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: OCT4 Antibody [NBP2-15053] - Whole cell extracts (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with OCT4 antibody at a dilution of 1:50000.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: OCT4 Antibody [NBP2-15053] - A. 30 ug human ESC whole cell extract 12% SDS-PAGE Oct4 antibody dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: OCT4 Antibody [NBP2-15053] - Paraffin-embedded Cal27 xenograft, using antibody at 1:500 dilution.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: OCT4 Antibody [NBP2-15053] - Oct4 antibody detects Oct4 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Oct4 stained by Oct4 antibody diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: OCT4 Antibody [NBP2-15053] - Human embryonic stem cells, staining with OCT3/4 or rabbit IgG (black).