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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [2]
- Other assay [1]
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- Product number
- MA1-104-D488 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- OCT4 Monoclonal Antibody (9B7), DyLight™ 488
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- MA1-104-D488 detects Oct4/Pou5F1 in human iPS samples. MA1-104-D488 has been successfully used in ICC/IF applications.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Antibody clone number
- 9B7
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C, do not freeze
Submitted references PACAP and PAC1R are differentially expressed in motor cortex of amyotrophic lateral sclerosis patients and support survival of iPSC-derived motor neurons.
Bonaventura G, Iemmolo R, D'Amico AG, La Cognata V, Costanzo E, Zappia M, D'Agata V, Conforti FL, Aronica E, Cavallaro S
Journal of cellular physiology 2018 Apr;233(4):3343-3351
Journal of cellular physiology 2018 Apr;233(4):3343-3351
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Oct4/Pou5F1 (green) shows nuclear staining in human HEL 11.4 iPS cells, indicating pluripotency. Oct4/Pou5F1 staining (left panel), DAPI staining (middle panel) and an overlay image of Oct4/Pou5F1 with DAPI (right panel) are shown. HEL 11.4 iPS cells were grown on matrigel-coated chamber slides and fixed with 4% formaldehyde for 15 minutes prior to staining. Cells were probed with a DyLight 488-conjugated Oct4/Pou5F1 monoclonal antibody (Product # MA1-104-D488) in 0.1% Tween-PBS at a dilution of 1:20, and incubated overnight at 4C. Images were taken at 20X magnification.
- Conjugate
- Green dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of OCT-4 was performed using 70% confluent log phase NTERA-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with OCT-4 Mouse Monoclonal Antibody (Product # MA1-104-D488) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e shows OCT-4 negative cell line HeLa with no signal. Panel f represents control cells with Isotype control to assess background. The images were captured at 60X magnification.
- Conjugate
- Green dye
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Green dye
