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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- MA1-104-HRP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- OCT4 Monoclonal Antibody (9B7), HRP
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- MA1-104-HRP detects Oct4/Pou5F1 in human embryonic carcinoma cells.
- Conjugate
- Horseradish Peroxidase
- Antibody clone number
- 9B7
- Concentration
- 1 mg/mL
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of Oct4/Pou5F1 was performed by loading 50 µg of NCCIT and negative control HeLa cell lysates per well onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane. The membrane was blocked, and probed with an HRP-conjugated Oct4/Pou5F1 monoclonal antibody (Product # MA1-104-HRP) at a dilution of 1:1000 overnight at 4C. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34075).
- Conjugate
- Horseradish Peroxidase
