MA1-104-HRP

antibody from Invitrogen Antibodies

Targeting: POU5F1 MGC22487, OCT3, Oct4, OTF3

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-104-HRP

Provider

Invitrogen Antibodies

Product name

OCT4 Monoclonal Antibody (9B7), HRP

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

MA1-104-HRP detects Oct4/Pou5F1 in human embryonic carcinoma cells. By Western blot, this antibody detects a band at 40 kDa in human embryonal carcinoma cells, but not in negative control cells.

Reactivity

Human

Host

Mouse

Conjugate

Horseradish Peroxidase

Isotype

IgG

Antibody clone number

9B7

Vial size

100 µL

Concentration

1 mg/mL

Storage

4° C, do not freeze

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Oct4/Pou5F1 was performed by loading 50 µg of NCCIT and negative control HeLa cell lysates per well onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane. The membrane was blocked, and probed with an HRP-conjugated Oct4/Pou5F1 monoclonal antibody (Product # MA1-104-HRP) at a dilution of 1:1000 overnight at 4C. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34075).

Conjugate

Horseradish Peroxidase