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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- TA319164 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal anti-Oct-4 antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal anti-Oct-4 antibody
- Reactivity
- Horse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- POU5F1
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- 1.0 mg/mL
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Supportive validation
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- OriGene (provider)
- Main image
- Experimental details
- WB using Anti-Oct4 antibody shows detection of endogenous Oct4 in mouse embryonic fibroblast (MEF) cell lysate (lane 1) and HeLa nuclear extract (HN) (lane 2). The band at ~39 kDa (arrow head) corresponds to Oct4. After transfer, the membrane was blocked with 5% BSA. Primary antibody was used at a 1:1,000 dilution in PBS containing 5% BSA. The specificity of the antibody was confirmed by peptide competition which blocks reaction of the antibody with Oct4 (lanes 3 and 4, respectively).
- Validation comment
- WB
