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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- TA324706 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal OCT4 (OCT3) Antibody (E125)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal OCT4 (OCT3) Antibody (E125)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- POU5F1
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- 2.0 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of OCT3/4 Antibody (E125) in HL60 cell line lysates (35ug/lane). OCT3/4 (arrow) was detected using the purified Pab.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IF image of SY5Y cells stained with TA324706 OCT4 (E125) antibody. SY5Y cells were incubated with TA324706 OCT4 (E125) primary antibody (1:500, 2 h at RT). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin . Nuclei were counterstained with Hoechst 33342 (blue). OCT4 immunoreactivity is localized mainly to the nuclei and also to the cytoplasm.
- Validation comment
- IF
