PAB10296

antibody from Abnova Corporation

Targeting: POU5F1 MGC22487, OCT3, Oct4, OTF3

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB10296

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB10296, RRID:AB_1710510

Product name

POU5F1 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of POU5F1.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Oct-4 mRNA and protein expression during human preimplantation development.

Cauffman G, Van de Velde H, Liebaers I, Van Steirteghem A
Molecular human reproduction 2005 Mar;11(3):173-81

---

Oct-4-expressing cells in human amniotic fluid: a new source for stem cell research?

Prusa AR, Marton E, Rosner M, Bernaschek G, Hengstschläger M
Human reproduction (Oxford, England) 2003 Jul;18(7):1489-93

---

High Oct-ane fuel powers the stem cell.

Donovan PJ
Nature genetics 2001 Nov;29(3):246-7

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot using POU5F1 polyclonal antibody (Cat # PAB10296) shows detection of endogenous POU5F1 in mouse embryonic fibroblast (MEF) cell lysate (lane 1) and HeLa nuclear extract (HN) (lane 2).The band at ~39 kDa (arrowhead) corresponds to POU5F1.After transfer, the membrane was blocked with 5% BSA.Primary antibody was used at a 1 : 1,000 dilution in PBS containing 5% BSA.The specificity of the antibody was confirmed by peptide competition which blocks reaction of the antibody with POU5F1 (lanes 3 and 4, respectively).