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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- TA500035 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500035, RRID:AB_2167722
- Product name
- OCT4 mouse monoclonal antibody, clone OTI9B7 (formerly 9B7)
- Antibody type
- Monoclonal
- Description
- OCT4 mouse monoclonal antibody, clone OTI9B7 (formerly 9B7)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- POU5F1
- Isotype
- IgG
- Antibody clone number
- OTI9B7
- Vial size
- 100 µl
- Concentration
- 0.25 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OCT4 (RC211998, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OCT4.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 1 cell line by using anti-OCT4 monoclonal antibody at 1:200 dilution.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-OCT4 mouse monoclonal antibody (TA500035) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY OCT4(RC211998).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-OCT4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500035)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-OCT4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500035)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-OCT4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500035)
- Validation comment
- IHC
