Antibody data
- Antibody Data
- Antigen structure
- References [25]
- Comments [0]
- Validations
- Western blot [2]
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- AF1759 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Oct-3/4 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Oct-3/4 in direct ELISAs and Western blots.
- Reactivity
- Human, Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references A multiplexed gRNA piggyBac transposon system facilitates efficient induction of CRISPRi and CRISPRa in human pluripotent stem cells.
Functional characterization of human pluripotent stem cell-derived cortical networks differentiated on laminin-521 substrate: comparison to rat cortical cultures.
SOX2 and SOX9 are markers of clinically aggressive disease in metastatic high-grade serous carcinoma.
The BAF and PRC2 Complex Subunits Dpf2 and Eed Antagonistically Converge on Tbx3 to Control ESC Differentiation.
O-GlcNAc transferase activates stem-like cell potential in hepatocarcinoma through O-GlcNAcylation of eukaryotic initiation factor 4E.
Small non-coding RNA landscape of extracellular vesicles from human stem cells.
Disrupted alternative splicing for genes implicated in splicing and ciliogenesis causes PRPF31 retinitis pigmentosa.
Generation of nine induced pluripotent stem cell lines as an ethnic diversity panel.
Dinaciclib potently suppresses MCL-1 and selectively induces the cell death in human iPS cells without affecting the viability of cardiac tissue.
Generation of a TLE3 heterozygous knockout human embryonic stem cell line using CRISPR-Cas9.
Generation of a TLE1 homozygous knockout human embryonic stem cell line using CRISPR-Cas9.
Brain tissue banking for stem cells for our future.
Semaphorin 3A induces mesenchymal-stem-like properties in human periodontal ligament cells.
Effects of antioxidants on the quality and genomic stability of induced pluripotent stem cells.
Pluripotent stem cells derived from mouse and human white mature adipocytes.
Genome-wide chromatin interactions of the Nanog locus in pluripotency, differentiation, and reprogramming.
Generation of corneal epithelial cells from induced pluripotent stem cells derived from human dermal fibroblast and corneal limbal epithelium.
A novel role for an RNA polymerase III subunit POLR3G in regulating pluripotency in human embryonic stem cells.
Glial cell line-derived neurotrophic factor and endothelial cells promote self-renewal of rabbit germ cells with spermatogonial stem cell properties.
SIP1 mediates cell-fate decisions between neuroectoderm and mesendoderm in human pluripotent stem cells.
miR-302b maintains "stemness" of human embryonal carcinoma cells by post-transcriptional regulation of Cyclin D2 expression.
Regulation of self-renewal and pluripotency by Sox2 in human embryonic stem cells.
Differential requirements for hematopoietic commitment between human and rhesus embryonic stem cells.
A comparison of NIH-approved human ESC lines.
Controlled-rate freezing of human ES cells.
Hazelbaker DZ, Beccard A, Angelini G, Mazzucato P, Messana A, Lam D, Eggan K, Barrett LE
Scientific reports 2020 Jan 20;10(1):635
Scientific reports 2020 Jan 20;10(1):635
Functional characterization of human pluripotent stem cell-derived cortical networks differentiated on laminin-521 substrate: comparison to rat cortical cultures.
Hyvärinen T, Hyysalo A, Kapucu FE, Aarnos L, Vinogradov A, Eglen SJ, Ylä-Outinen L, Narkilahti S
Scientific reports 2019 Nov 20;9(1):17125
Scientific reports 2019 Nov 20;9(1):17125
SOX2 and SOX9 are markers of clinically aggressive disease in metastatic high-grade serous carcinoma.
Sherman-Samis M, Onallah H, Holth A, Reich R, Davidson B
Gynecologic oncology 2019 Jun;153(3):651-660
Gynecologic oncology 2019 Jun;153(3):651-660
The BAF and PRC2 Complex Subunits Dpf2 and Eed Antagonistically Converge on Tbx3 to Control ESC Differentiation.
Zhang W, Chronis C, Chen X, Zhang H, Spalinskas R, Pardo M, Chen L, Wu G, Zhu Z, Yu Y, Yu L, Choudhary J, Nichols J, Parast MM, Greber B, Sahlén P, Plath K
Cell stem cell 2019 Jan 3;24(1):138-152.e8
Cell stem cell 2019 Jan 3;24(1):138-152.e8
O-GlcNAc transferase activates stem-like cell potential in hepatocarcinoma through O-GlcNAcylation of eukaryotic initiation factor 4E.
Cao B, Duan M, Xing Y, Liu C, Yang F, Li Y, Yang T, Wei Y, Gao Q, Jiang J
Journal of cellular and molecular medicine 2019 Apr;23(4):2384-2398
Journal of cellular and molecular medicine 2019 Apr;23(4):2384-2398
Small non-coding RNA landscape of extracellular vesicles from human stem cells.
Kaur S, Abu-Shahba AG, Paananen RO, Hongisto H, Hiidenmaa H, Skottman H, Seppänen-Kaijansinkko R, Mannerström B
Scientific reports 2018 Oct 19;8(1):15503
Scientific reports 2018 Oct 19;8(1):15503
Disrupted alternative splicing for genes implicated in splicing and ciliogenesis causes PRPF31 retinitis pigmentosa.
Buskin A, Zhu L, Chichagova V, Basu B, Mozaffari-Jovin S, Dolan D, Droop A, Collin J, Bronstein R, Mehrotra S, Farkas M, Hilgen G, White K, Pan KT, Treumann A, Hallam D, Bialas K, Chung G, Mellough C, Ding Y, Krasnogor N, Przyborski S, Zwolinski S, Al-Aama J, Alharthi S, Xu Y, Wheway G, Szymanska K, McKibbin M, Inglehearn CF, Elliott DJ, Lindsay S, Ali RR, Steel DH, Armstrong L, Sernagor E, Urlaub H, Pierce E, Lührmann R, Grellscheid SN, Johnson CA, Lako M
Nature communications 2018 Oct 12;9(1):4234
Nature communications 2018 Oct 12;9(1):4234
Generation of nine induced pluripotent stem cell lines as an ethnic diversity panel.
Gao X, Yourick JJ, Sprando RL
Stem cell research 2018 Aug;31:193-196
Stem cell research 2018 Aug;31:193-196
Dinaciclib potently suppresses MCL-1 and selectively induces the cell death in human iPS cells without affecting the viability of cardiac tissue.
Alsayegh K, Matsuura K, Sekine H, Shimizu T
Scientific reports 2017 Mar 31;7:45577
Scientific reports 2017 Mar 31;7:45577
Generation of a TLE3 heterozygous knockout human embryonic stem cell line using CRISPR-Cas9.
Bara AM, Messana A, Herring A, Hazelbaker DZ, Eggan K, Barrett LE
Stem cell research 2016 Sep;17(2):441-443
Stem cell research 2016 Sep;17(2):441-443
Generation of a TLE1 homozygous knockout human embryonic stem cell line using CRISPR-Cas9.
Herring A, Messana A, Bara AM, Hazelbaker DZ, Eggan K, Barrett LE
Stem cell research 2016 Sep;17(2):430-432
Stem cell research 2016 Sep;17(2):430-432
Brain tissue banking for stem cells for our future.
Palmero E, Palmero S, Murrell W
Scientific reports 2016 Dec 19;6:39394
Scientific reports 2016 Dec 19;6:39394
Semaphorin 3A induces mesenchymal-stem-like properties in human periodontal ligament cells.
Wada N, Maeda H, Hasegawa D, Gronthos S, Bartold PM, Menicanin D, Fujii S, Yoshida S, Tomokiyo A, Monnouchi S, Akamine A
Stem cells and development 2014 Sep 15;23(18):2225-36
Stem cells and development 2014 Sep 15;23(18):2225-36
Effects of antioxidants on the quality and genomic stability of induced pluripotent stem cells.
Luo L, Kawakatsu M, Guo CW, Urata Y, Huang WJ, Ali H, Doi H, Kitajima Y, Tanaka T, Goto S, Ono Y, Xin HB, Hamano K, Li TS
Scientific reports 2014 Jan 21;4:3779
Scientific reports 2014 Jan 21;4:3779
Pluripotent stem cells derived from mouse and human white mature adipocytes.
Jumabay M, Abdmaulen R, Ly A, Cubberly MR, Shahmirian LJ, Heydarkhan-Hagvall S, Dumesic DA, Yao Y, Boström KI
Stem cells translational medicine 2014 Feb;3(2):161-71
Stem cells translational medicine 2014 Feb;3(2):161-71
Genome-wide chromatin interactions of the Nanog locus in pluripotency, differentiation, and reprogramming.
Apostolou E, Ferrari F, Walsh RM, Bar-Nur O, Stadtfeld M, Cheloufi S, Stuart HT, Polo JM, Ohsumi TK, Borowsky ML, Kharchenko PV, Park PJ, Hochedlinger K
Cell stem cell 2013 Jun 6;12(6):699-712
Cell stem cell 2013 Jun 6;12(6):699-712
Generation of corneal epithelial cells from induced pluripotent stem cells derived from human dermal fibroblast and corneal limbal epithelium.
Hayashi R, Ishikawa Y, Ito M, Kageyama T, Takashiba K, Fujioka T, Tsujikawa M, Miyoshi H, Yamato M, Nakamura Y, Nishida K
PloS one 2012;7(9):e45435
PloS one 2012;7(9):e45435
A novel role for an RNA polymerase III subunit POLR3G in regulating pluripotency in human embryonic stem cells.
Wong RC, Pollan S, Fong H, Ibrahim A, Smith EL, Ho M, Laslett AL, Donovan PJ
Stem cells (Dayton, Ohio) 2011 Oct;29(10):1517-27
Stem cells (Dayton, Ohio) 2011 Oct;29(10):1517-27
Glial cell line-derived neurotrophic factor and endothelial cells promote self-renewal of rabbit germ cells with spermatogonial stem cell properties.
Kubota H, Wu X, Goodyear SM, Avarbock MR, Brinster RL
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2011 Aug;25(8):2604-14
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2011 Aug;25(8):2604-14
SIP1 mediates cell-fate decisions between neuroectoderm and mesendoderm in human pluripotent stem cells.
Chng Z, Teo A, Pedersen RA, Vallier L
Cell stem cell 2010 Jan 8;6(1):59-70
Cell stem cell 2010 Jan 8;6(1):59-70
miR-302b maintains "stemness" of human embryonal carcinoma cells by post-transcriptional regulation of Cyclin D2 expression.
Lee NS, Kim JS, Cho WJ, Lee MR, Steiner R, Gompers A, Ling D, Zhang J, Strom P, Behlke M, Moon SH, Salvaterra PM, Jove R, Kim KS
Biochemical and biophysical research communications 2008 Dec 12;377(2):434-440
Biochemical and biophysical research communications 2008 Dec 12;377(2):434-440
Regulation of self-renewal and pluripotency by Sox2 in human embryonic stem cells.
Fong H, Hohenstein KA, Donovan PJ
Stem cells (Dayton, Ohio) 2008 Aug;26(8):1931-8
Stem cells (Dayton, Ohio) 2008 Aug;26(8):1931-8
Differential requirements for hematopoietic commitment between human and rhesus embryonic stem cells.
Rajesh D, Chinnasamy N, Mitalipov SM, Wolf DP, Slukvin I, Thomson JA, Shaaban AF
Stem cells (Dayton, Ohio) 2007 Feb;25(2):490-9
Stem cells (Dayton, Ohio) 2007 Feb;25(2):490-9
A comparison of NIH-approved human ESC lines.
Ware CB, Nelson AM, Blau CA
Stem cells (Dayton, Ohio) 2006 Dec;24(12):2677-84
Stem cells (Dayton, Ohio) 2006 Dec;24(12):2677-84
Controlled-rate freezing of human ES cells.
Ware CB, Nelson AM, Blau CA
BioTechniques 2005 Jun;38(6):879-80, 882-3
BioTechniques 2005 Jun;38(6):879-80, 882-3
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Oct-3/4 by Simple WesternTM. Detection of Human Oct-3/4 by Simple WesternTM.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human and Mouse Oct-3/4 by Western Blot. Detection of Human and Mouse Oct-3/4 by Western Blot.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Oct-3/4-regulated Genes by Chromatin Immunoprecipitation. Detection of Oct-3/4-regulated Genes by Chromatin Immunoprecipitation.