A00174-1

antibody from Boster Biological Technology

Targeting: POU5F1 MGC22487, OCT3, Oct4, OTF3

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

A00174-1

Provider

Boster Biological Technology

Product name

Anti-Oct4/POU5F1 Antibody

Antibody type

Polyclonal

Description

Rabbit IgG polyclonal antibody for Oct4/POU5F1 detection. Tested with WB, FCM, Direct ELISA in Human;Mouse;Rat.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

100μg/vial

Concentration

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

References

Submitted references

Angiotensin (1-7) reverses glucose-induced islet β cell dedifferentiation by Wnt/β-catenin/FoxO1 signalling pathway.

Guo D, He J, Guo H, Song G, Peng L, An M, Wang C
Endokrynologia Polska 2023;74(5):544-552

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LncRNA LINC00662 Exerts an Oncogenic Effect on Osteosarcoma by the miR-16-5p/ITPR1 Axis.

Yu M, Lu W, Cao Z, Xuan T
Journal of oncology 2021;2021:8493431

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Insulin-like growth factor binding protein 4 enhances cardiomyocytes induction in murine-induced pluripotent stem cells.

Xue Y, Yan Y, Gong H, Fang B, Zhou Y, Ding Z, Yin P, Zhang G, Ye Y, Yang C, Ge J, Zou Y
Journal of cellular biochemistry 2014 Sep;115(9):1495-504

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Knockdown of OCT4 suppresses the growth and invasion of pancreatic cancer cells through inhibition of the AKT pathway.

Lin H, Sun LH, Han W, He TY, Xu XJ, Cheng K, Geng C, Su LD, Wen H, Wang XY, Chen QL
Molecular medicine reports 2014 Sep;10(3):1335-42

Flow cytometry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Flow Cytometry analysis of 293T cells using anti-POU5F1 antibody (A00174-1). Overlay histogram showing 293T cells stained with A00174-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-POU5F1 Antibody (A00174-1,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Additional image