- MA5-14845 - Invitrogen Antibodies POU5F1 antibody

Submitted references Inhibition of SARS-CoV-2 infection in human iPSC-derived cardiomyocytes by targeting the Sigma-1 receptor disrupts cytoarchitecture and beating.

Salerno JA, Torquato T, Temerozo JR, Goto-Silva L, Karmirian K, Mendes MA, Sacramento CQ, Fintelman-Rodrigues N, Souza LRQ, Ornelas IM, Veríssimo CP, Aragão LGHS, Vitória G, Pedrosa CSG, da Silva Gomes Dias S, Cardoso Soares V, Puig-Pijuan T, Salazar V, Dariolli R, Biagi D, Furtado DR, Barreto Chiarini L, Borges HL, Bozza PT, Zaluar P Guimarães M, Souza TML, Rehen SK
PeerJ 2021;9:e12595

Therapeutic manipulation of IKBKAP mis-splicing with a small molecule to cure familial dysautonomia.

Ajiro M, Awaya T, Kim YJ, Iida K, Denawa M, Tanaka N, Kurosawa R, Matsushima S, Shibata S, Sakamoto T, Studer L, Krainer AR, Hagiwara M
Nature communications 2021 Jul 23;12(1):4507

Altered cytoskeletal arrangement in induced pluripotent stem cells (iPSCs) and motor neurons from patients with riboflavin transporter deficiency.

Niceforo A, Marioli C, Colasuonno F, Petrini S, Massey K, Tartaglia M, Bertini E, Moreno S, Compagnucci C
Disease models & mechanisms 2021 Jan 19;14(2)

Human Cardiac Organoids for Modeling Genetic Cardiomyopathy.

Filippo Buono M, von Boehmer L, Strang J, Hoerstrup SP, Emmert MY, Nugraha B
Cells 2020 Jul 20;9(7)

Head and Neck Cancer Stem Cell-Enriched Spheroid Model for Anticancer Compound Screening.

Goričan L, Gole B, Potočnik U
Cells 2020 Jul 16;9(7)

No comments: Submit comment

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Oct-4A in mouse embryonic stem cells growing on mouse embryonic fibroblast feeder cells, using an oct-4A monoclonal antibody (Product # MA5-14845) (green). Actin filaments are labeled with a fluorescent red phalloidin.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Oct-4A in NTERA2 cells, using an oct-4A monoclonal antibody (Product # MA5-14845) (green). Actin filaments are labeled with a fluorescent red phalloidin.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometric analysis of Oct-4A in HeLa cells (blue) and NCCIT cells (green) using a Oct-4A monoclonal antibody (Product # MA5-14845).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2 Characterization of cardiomyocytes derived from WTSIi020-A and UKKi025-A. ( A , B ) Flow cytometry analysis of cells positive for TNNT2 and OCT4 at the beginning (day 0) and the end (day 15) of differentiation in CDM3. ( C , D ) Median fluorescence intensities of TNNT2 + and OCT4 + populations at day 0 and 15 of the CDM3 protocol. n = 3 biological replicates. ( E , F ) Heatmap plots representing RT-qPCR for markers of pluripotency (NANOG, OCT4, SOX2, KLF4, MYC), cardiac progenitors (NKX2.5) and cardiomyocytes (TNNT2, MHY6, MHY7) on day 0 and day 15. n = 3 independent biological replicates performed in technical triplicates. ( G ) Immunofluorescent staining images of cardiomyocytes derived from WTSIi020-A and UKKi025-A. WTSIi020-A CMs show clear formation of immature sarcomeric-like structure while UKKi025-A CMs display a disrupted structure and rather enlarged cell morphology (marked with *). Significance level A : **** p oct4 < 0.001; * p tnnt2 < 0.023; B : *** p oct4 = 0.0002; **** p tnnt2 < 0.0001; C : ** p oct4 = 0.0015; **** p tnnt2 < 0.0001; D : * p oct4 = 0.0142; **** p tnnt2 < 0.0001; E : p

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 1. Patient-specific model validation and characterization of RTD iPSCs. (A) iPSCs from two RTD patients express pluripotentcy markers, as assessed by immunofluorescence for SSEA4, SOX-2 (in green), OCT-4 and TRA 1-60 (in red). (B) Phase contrast images show an absence of typical colonies in RTD iPSCs compared to Ctrl. (C) OCT4 and KLF4 gene expression evaluated by RT-qPCR, using ACTB as a reference standard. Similar values of Ctrl, RTD P1 and RTD P2 iPSCs are detected. (D) MTT assay for cell viability. The percentage of viable cells in P1 and P2 iPSC cultures are comparable to Ctrl. Data are mean+-s.e.m. Scale bars: 50 mum (A); 20 mum (B).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 4 Protein expression of stem markers. The protein expression of five different stem markers was assessed in the FaDu adherent cell line and 7-day-old SCESM spheroids by Western blot and flow cytometry. ( a - d ) Protein expression analyzed by flow cytometry. Normalized fluorescence intensities of selected proteins are presented, and corresponding flow cytometry visible light and fluorescent photos are attached. Normalized mean values +- SE ( n >= 5, N >= 2000) are presented (**** p < 0.0001 vs. control (adh)); ( a ) CD44 protein expression; ( b ) CD73 protein expression; ( c ) CD90 protein expression; ( d ) CD133 protein expression; ( e ) OCT4 and tubulin protein expression analyzed by Western blot. Western blot membrane photos showing OCT4 and tubulin and a graph depicting normalized OCT4 protein expression analysis from Western blots are presented. Bars represent normalized mean values +- SE ( n >= 3). B: blank; adh: adherent cell line; S: SCESM.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 5 Comparative analysis of SCESM spheroids and multi-cellular tumor spheroids (MCTSs). ( a ) Graph representing SCESM spheroid and MCTS mean diameter increase. Spheroids were photographed on days 2, 4, and 7 and representative phase-contrast microscope images of SCESM spheroids and MCTSs on days 2, 4, and 7 are displayed under the graph. Mean values +- SE ( n >= 8) are presented. Scale bars correspond to 400 um; ( b - e ) Protein expression of four different stem markers was assessed in SCESM spheroids and MCTSs by flow cytometry. Normalized fluorescence intensities of selected proteins are presented and corresponding flow cytometry visible light and fluorescent photos are attached ( n >= 5, N >= 2000); (b) CD44 protein expression; ( c ) CD73 protein expression; ( d ) CD90 protein expression; ( e ) CD133 protein expression; ( f ) OCT4 and tubulin protein expressions were assessed in SCESM spheroids and MCTSs by Western blot analysis. Western blot membrane photo showing OCT4 and tubulin and graph depicting normalized OCT4 protein expression analysis from Western blots (using Image Lab software) are presented ( n >= 3); ( g - i ) Relative gene expression of three additional stem markers assessed in 7-day-old MCTSs and SCESM spheroids; Normalized mean values +- SE ( n >= 5) are presented; ( g ) Relative gene expression of ALDH1A1 ; ( h ) Relative gene expression of NANOG ; ( i ) Relative gene expression of SOX2 . (* p < 0.05, **** p < 0.0001 vs. control (MCTS)). B: blank;

MA5-14845

Antibody data