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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- AP09402PU-S - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP09402PU-S, RRID:AB_2035870
- Product name
- anti OCT3/4
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide corresponding to an internal region of human Oct-4 protein
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 25 µl
- Concentration
- 1.0 mg/ml (by UV absorbance at 280 nm)
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Supportive validation
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- Acris Antibodies GmbH (provider)
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- Experimental details
- Western blot using affinity purified anti-Oct4 antibody shows detection of endogenous Oct4 in mouse embryonic fibroblast (MEF) cell lysate (lane 1) and HeLa nuclear extract (HN) (lane 2). The band at ~39 kDa (arrow head) corresponds to Oct4. After transfer, the membrane was blocked with 5% BSA. Primary antibody was used at a 1:1,000 dilution in PBS containing 5% BSA. The specificity of the antibody was confirmed by peptide competition which blocks reaction of the antibody with Oct4 (lanes 3 and 4, respectively).
