Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Other assay [1]
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Validation data
Reference
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- Product number
- V2569 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- HLA-ABC Antibody (MHC I)
- Antibody type
- Monoclonal
- Antigen
- Human peripheral blood leukocytes were used as the immunogen for the HLA-ABC antibody.
- Description
- Protein G purified antibody
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 246-B8.E7
- Vial size
- 20 µg, 100 µg (with sodium azide), 100 µg (without sodium azide), 7 ml (prediluted for IHC use, if applicable)
- Storage
- Store the HLA-ABC antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Submitted references HLA class I antibodies trigger increased adherence of monocytes to endothelial cells by eliciting an increase in endothelial P-selectin and, depending on subclass, by engaging FcγRs.
Valenzuela NM, Mulder A, Reed EF
Journal of immunology (Baltimore, Md. : 1950) 2013 Jun 15;190(12):6635-50
Journal of immunology (Baltimore, Md. : 1950) 2013 Jun 15;190(12):6635-50
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of ThP-1 cell lysate with HLA-ABC antibody. Expected molecular weight of A/B/C: 40-41kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE Analysis of Purified, BSA-Free HLA-ABC Antibody (clone 246-B8.E7). Confirmation of Integrity and Purity of the Antibody.