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- Product number
- 16-5788-025 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MICA/B Monoclonal Antibody (6D4), Functional Grade, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The 6D4 monoclonal antibody reacts with the human major histocompatibility complex (MHC) class I chain-related (MIC), MICA and MICB proteins. MICA and MICB are related proteins of 83% amino acid similarity, and show homology with classical human leukocyte antigen (HLA) molecules. The structure of MICA and MICB are similar to classical HLA class I chains, however they do not bind beta2 microglobulin or bind peptide typical of HLA class I. MICA and MICB are expressed on the cell surface of endothelial cells, fibroblasts, gastric epithelium and PHA-stimulated T cells, and act as a ligand for NKGD2 expressed on the surface of NK cells, gammadelta T cells and alpha beta CD8+ T cells. There is evidence to suggest that human cytomegalovirus (HCMV) subverts NK cell detection by inhibiting the function of MICB. Furthermore, MICA and MICB expression has been detected in several epithelial tumours isolated from breast, lung, ovary, prostate, colon and kidney.
- Antibody clone number
- 6D4
- Concentration
- 1 mg/mL
Submitted references Low dose gemcitabine increases the cytotoxicity of human Vγ9Vδ2 T cells in bladder cancer cells in vitro and in an orthotopic xenograft model.
Role of NKG2D in cytokine-induced killer cells against lung cancer.
Protective roles of epithelial cells in the survival of adult T-cell leukemia/lymphoma cells.
Evidence for no relevance of anti-major histocompatibility complex class I-related chain a antibodies in liver transplantation.
Shimizu T, Tomogane M, Miyashita M, Ukimura O, Ashihara E
Oncoimmunology 2018;7(5):e1424671
Oncoimmunology 2018;7(5):e1424671
Role of NKG2D in cytokine-induced killer cells against lung cancer.
Yin X, Lu X, Xiuwen Z, Min Z, Xiao R, Mao Z, Zhang Q
Oncology letters 2017 May;13(5):3139-3143
Oncology letters 2017 May;13(5):3139-3143
Protective roles of epithelial cells in the survival of adult T-cell leukemia/lymphoma cells.
Miyatake Y, Oliveira AL, Jarboui MA, Ota S, Tomaru U, Teshima T, Hall WW, Kasahara M
The American journal of pathology 2013 May;182(5):1832-42
The American journal of pathology 2013 May;182(5):1832-42
Evidence for no relevance of anti-major histocompatibility complex class I-related chain a antibodies in liver transplantation.
Uzunel M, Kasimu H, Joshi M, Ge X, Liu J, Xu B, Jaksch M, Jorns C, Nowak G, Sumitran-Holgersson S
Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society 2008 Dec;14(12):1793-802
Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society 2008 Dec;14(12):1793-802
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- Figure 4. Changes of MICA/B expression in UBC cells following treatment with gemcitabine and in vitro cytotoxicity assay in MICA/B-knockdown UBC cells. (A) MICA/B expression in T24 and TCCSUP cells before and after ZOL, cisplatin, gemcitabine, and both ZOL and gemcitabine treatment. All agents were used at a concentration of 5 muM for 24 h. MICA/B expression was examined by FACS. A representative histogram is shown. Red histogram: background control; blue: no treatment; orange: agent treatment. (B) Median fluorescence intensity (MFI) of MICA/B expression in each agent-treated sample was quantified and normalized to that of the untreated control sample. Data represent the mean +- SD of triplicate wells. (C) T24 and TCCSUP cells were treated with 5 muM gemcitabine for 24 h. MICA and MICB mRNA transcripts were examined by quantitative RT-PCR. (D) Effects of MICA/B small interfering RNA (siMICA/B). (E) In vitro cytotoxicity assay was performed on MICA/B-knockdown UBC cells. (F) In vitro cytotoxicity assay was performed on UBC cells in the presence of anti-MICA/B blocking mAb or isotype control. (G) In vitro cytotoxicity assay was performed on UBC cells in the presence of anti-NKG2D blocking mAb or isotype control. Data represent the mean +- SD of triplicate wells. Statistical significance is displayed as ** for P < 0.01.
