NBP2-24959
antibody from Novus Biologicals
Targeting: TLR5
FLJ10052, MGC126430, MGC126431, SLEB1, TIL3
Antibody data
- Antibody Data
- Antigen structure
- References [18]
- Comments [0]
- Validations
- Flow cytometry [3]
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- Product number
- NBP2-24959 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal TLR5 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human, Mouse, Canine
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C in the dark.
Submitted references Vaccination against canine leishmaniosis increases the phagocytic activity, nitric oxide production and expression of cell activation/migration molecules in neutrophils and monocytes.
Dendritic cells induce Th2-mediated airway inflammatory responses to house dust mite via DNA-dependent protein kinase.
Flagellin/TLR5 signalling activates renal collecting duct cells and facilitates invasion and cellular translocation of uropathogenic Escherichia coli.
Characterization of the Toll-like receptor expression profile in human multiple myeloma cells.
Regulation of Toll-like receptor 5 gene expression and function on mucosal dendritic cells.
The K5 capsule of Escherichia coli strain Nissle 1917 is important in stimulating expression of Toll-like receptor 5, CD14, MyD88, and TRIF together with the induction of interleukin-8 expression via the mitogen-activated protein kinase pathway in epithelial cells.
Toll-like receptor triggering in cord blood mesenchymal stem cells.
Toll-like receptor agonists induce inflammation and cell death in a model of head and neck squamous cell carcinomas.
Expression patterns and functions of toll-like receptors in mouse sertoli cells.
Human lymphatic endothelial cells express multiple functional TLRs.
Human lymphatic endothelial cells express multiple functional TLRs.
Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry.
Intracellular signaling mechanisms regulating toll-like receptor-mediated activation of eosinophils.
Modulation of neonatal microbial recognition: TLR-mediated innate immune responses are specifically and differentially modulated by human milk.
Modulation of neonatal microbial recognition: TLR-mediated innate immune responses are specifically and differentially modulated by human milk.
Toll-like receptors in cellular subsets of human tonsil T cells: altered expression during recurrent tonsillitis.
Human CD4+ T cells express TLR5 and its ligand flagellin enhances the suppressive capacity and expression of FOXP3 in CD4+CD25+ T regulatory cells.
Human CD4+ T cells express TLR5 and its ligand flagellin enhances the suppressive capacity and expression of FOXP3 in CD4+CD25+ T regulatory cells.
Moreira ML, Costa-Pereira C, Alves ML, Marteleto BH, Ribeiro VM, Peruhype-Magalhães V, Giunchetti RC, Martins-Filho OA, Araújo MS
Veterinary parasitology 2016 Apr 15;220:33-45
Veterinary parasitology 2016 Apr 15;220:33-45
Dendritic cells induce Th2-mediated airway inflammatory responses to house dust mite via DNA-dependent protein kinase.
Mishra A, Brown AL, Yao X, Yang S, Park SJ, Liu C, Dagur PK, McCoy JP, Keeran KJ, Nugent GZ, Jeffries KR, Qu X, Yu ZX, Levine SJ, Chung JH
Nature communications 2015 Feb 18;6:6224
Nature communications 2015 Feb 18;6:6224
Flagellin/TLR5 signalling activates renal collecting duct cells and facilitates invasion and cellular translocation of uropathogenic Escherichia coli.
Bens M, Vimont S, Ben Mkaddem S, Chassin C, Goujon JM, Balloy V, Chignard M, Werts C, Vandewalle A
Cellular microbiology 2014 Oct;16(10):1503-17
Cellular microbiology 2014 Oct;16(10):1503-17
Characterization of the Toll-like receptor expression profile in human multiple myeloma cells.
Abdi J, Mutis T, Garssen J, Redegeld F
PloS one 2013;8(4):e60671
PloS one 2013;8(4):e60671
Regulation of Toll-like receptor 5 gene expression and function on mucosal dendritic cells.
Feng T, Cong Y, Alexander K, Elson CO
PloS one 2012;7(4):e35918
PloS one 2012;7(4):e35918
The K5 capsule of Escherichia coli strain Nissle 1917 is important in stimulating expression of Toll-like receptor 5, CD14, MyD88, and TRIF together with the induction of interleukin-8 expression via the mitogen-activated protein kinase pathway in epithelial cells.
Hafez M, Hayes K, Goldrick M, Grencis RK, Roberts IS
Infection and immunity 2010 May;78(5):2153-62
Infection and immunity 2010 May;78(5):2153-62
Toll-like receptor triggering in cord blood mesenchymal stem cells.
van den Berk LC, Jansen BJ, Siebers-Vermeulen KG, Netea MG, Latuhihin T, Bergevoet S, Raymakers RA, Kögler G, Figdor CC, Adema GJ, Torensma R
Journal of cellular and molecular medicine 2009 Sep;13(9B):3415-26
Journal of cellular and molecular medicine 2009 Sep;13(9B):3415-26
Toll-like receptor agonists induce inflammation and cell death in a model of head and neck squamous cell carcinomas.
Rydberg C, Månsson A, Uddman R, Riesbeck K, Cardell LO
Immunology 2009 Sep;128(1 Suppl):e600-11
Immunology 2009 Sep;128(1 Suppl):e600-11
Expression patterns and functions of toll-like receptors in mouse sertoli cells.
Wu H, Wang H, Xiong W, Chen S, Tang H, Han D
Endocrinology 2008 Sep;149(9):4402-12
Endocrinology 2008 Sep;149(9):4402-12
Human lymphatic endothelial cells express multiple functional TLRs.
Pegu A, Qin S, Fallert Junecko BA, Nisato RE, Pepper MS, Reinhart TA
Journal of immunology (Baltimore, Md. : 1950) 2008 Mar 1;180(5):3399-405
Journal of immunology (Baltimore, Md. : 1950) 2008 Mar 1;180(5):3399-405
Human lymphatic endothelial cells express multiple functional TLRs.
Pegu A, Qin S, Fallert Junecko BA, Nisato RE, Pepper MS, Reinhart TA
Journal of immunology (Baltimore, Md. : 1950) 2008 Mar 1;180(5):3399-405
Journal of immunology (Baltimore, Md. : 1950) 2008 Mar 1;180(5):3399-405
Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry.
Burgener IA, Jungi TW
Veterinary immunology and immunopathology 2008 Jul 15;124(1-2):184-91
Veterinary immunology and immunopathology 2008 Jul 15;124(1-2):184-91
Intracellular signaling mechanisms regulating toll-like receptor-mediated activation of eosinophils.
Wong CK, Cheung PF, Ip WK, Lam CW
American journal of respiratory cell and molecular biology 2007 Jul;37(1):85-96
American journal of respiratory cell and molecular biology 2007 Jul;37(1):85-96
Modulation of neonatal microbial recognition: TLR-mediated innate immune responses are specifically and differentially modulated by human milk.
LeBouder E, Rey-Nores JE, Raby AC, Affolter M, Vidal K, Thornton CA, Labéta MO
Journal of immunology (Baltimore, Md. : 1950) 2006 Mar 15;176(6):3742-52
Journal of immunology (Baltimore, Md. : 1950) 2006 Mar 15;176(6):3742-52
Modulation of neonatal microbial recognition: TLR-mediated innate immune responses are specifically and differentially modulated by human milk.
LeBouder E, Rey-Nores JE, Raby AC, Affolter M, Vidal K, Thornton CA, Labéta MO
Journal of immunology (Baltimore, Md. : 1950) 2006 Mar 15;176(6):3742-52
Journal of immunology (Baltimore, Md. : 1950) 2006 Mar 15;176(6):3742-52
Toll-like receptors in cellular subsets of human tonsil T cells: altered expression during recurrent tonsillitis.
Mansson A, Adner M, Cardell LO
Respiratory research 2006 Feb 27;7:36
Respiratory research 2006 Feb 27;7:36
Human CD4+ T cells express TLR5 and its ligand flagellin enhances the suppressive capacity and expression of FOXP3 in CD4+CD25+ T regulatory cells.
Crellin NK, Garcia RV, Hadisfar O, Allan SE, Steiner TS, Levings MK
Journal of immunology (Baltimore, Md. : 1950) 2005 Dec 15;175(12):8051-9
Journal of immunology (Baltimore, Md. : 1950) 2005 Dec 15;175(12):8051-9
Human CD4+ T cells express TLR5 and its ligand flagellin enhances the suppressive capacity and expression of FOXP3 in CD4+CD25+ T regulatory cells.
Crellin NK, Garcia RV, Hadisfar O, Allan SE, Steiner TS, Levings MK
Journal of immunology (Baltimore, Md. : 1950) 2005 Dec 15;175(12):8051-9
Journal of immunology (Baltimore, Md. : 1950) 2005 Dec 15;175(12):8051-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TLR5 Antibody (85B152.5) [PE] [NBP2-24959] - Intracellular flow analysis of TLR5 in 10^6 human lymphocytes using 0.5 ugs of PE-conjugated antibody. The shaded histogram represents cells without antibody, green represents isotype control antibody, and red represents TLR5 antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TLR5 Antibody (85B152.5) [PE] [NBP2-24959] - Cell surface analysis of TLR5 in 10^6 RAW cells using 0.5 ugs of PE-conjugated antibody. The black histogram represents cells without antibody, green represents isotype control antibody, and red represents TLR5 antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TLR5 Antibody (85B152.5) [PE] [NBP2-24959] - A surface stain was performed on A431 cells with TLR5 [85B152.5] Antibody NBP2-24959 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.