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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- MAB6704 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal TLR5 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human TLR5 in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with recombinant human (rh) TLR1, 2, 3, 4, 7, 8, recombinant mouse TLR5, 6, or rhTIRAP is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- TLR5 in Human Intestine. TLR5 was detected in immersion fixed paraffin-embedded sections of human intestine using Mouse Anti-Human TLR5 Monoclonal Antibody (Catalog # MAB6704) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panels show a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of TLR5 in Human Human Peripheral Blood Monocytes by Flow Cytometry. Detection of TLR5 in Human Human Peripheral Blood Monocytes by Flow Cytometry.
