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LearnNBP1-97728
antibody from Novus Biologicals
Targeting: TLR5
FLJ10052, MGC126430, MGC126431, SLEB1, TIL3
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [34]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [5]
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- Product number
- NBP1-97728 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal TLR5 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human, Mouse, Canine
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references All-Trans Retinoic Acid Enhances Bacterial Flagellin-Stimulated Proinflammatory Responses in Human Monocyte THP-1 Cells by Upregulating CD14.
Toll-Like Receptor 1/2 and 5 Ligands Enhance the Expression of Cyclin D1 and D3 and Induce Proliferation in Mantle Cell Lymphoma.
Pseudomonas aeruginosa stimulates tyrosine phosphorylation of and TLR5 association with the MUC1 cytoplasmic tail through EGFR activation.
Vaccination against canine leishmaniosis increases the phagocytic activity, nitric oxide production and expression of cell activation/migration molecules in neutrophils and monocytes.
Inhibition of IRAK1/4 sensitizes T cell acute lymphoblastic leukemia to chemotherapies.
Dendritic cells induce Th2-mediated airway inflammatory responses to house dust mite via DNA-dependent protein kinase.
Flagellin/TLR5 signalling activates renal collecting duct cells and facilitates invasion and cellular translocation of uropathogenic Escherichia coli.
Prevention of graft-versus-host disease by adoptive T regulatory therapy is associated with active repression of peripheral blood Toll-like receptor 5 mRNA expression.
Characterization of the Toll-like receptor expression profile in human multiple myeloma cells.
Regulation of Toll-like receptor 5 gene expression and function on mucosal dendritic cells.
The K5 capsule of Escherichia coli strain Nissle 1917 is important in stimulating expression of Toll-like receptor 5, CD14, MyD88, and TRIF together with the induction of interleukin-8 expression via the mitogen-activated protein kinase pathway in epithelial cells.
Toll-like receptor triggering in cord blood mesenchymal stem cells.
Toll-like receptor agonists induce inflammation and cell death in a model of head and neck squamous cell carcinomas.
Expression patterns and functions of toll-like receptors in mouse sertoli cells.
Human lymphatic endothelial cells express multiple functional TLRs.
Human lymphatic endothelial cells express multiple functional TLRs.
Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry.
Intestinal bacteria trigger T cell-independent immunoglobulin A(2) class switching by inducing epithelial-cell secretion of the cytokine APRIL.
Intracellular signaling mechanisms regulating toll-like receptor-mediated activation of eosinophils.
Sertoli cells initiate testicular innate immune responses through TLR activation.
Sertoli cells initiate testicular innate immune responses through TLR activation.
Modulation of neonatal microbial recognition: TLR-mediated innate immune responses are specifically and differentially modulated by human milk.
Modulation of neonatal microbial recognition: TLR-mediated innate immune responses are specifically and differentially modulated by human milk.
Toll-like receptors in cellular subsets of human tonsil T cells: altered expression during recurrent tonsillitis.
Inhibition of neutrophil apoptosis by TLR agonists in whole blood: involvement of the phosphoinositide 3-kinase/Akt and NF-kappaB signaling pathways, leading to increased levels of Mcl-1, A1, and phosphorylated Bad.
Inhibition of neutrophil apoptosis by TLR agonists in whole blood: involvement of the phosphoinositide 3-kinase/Akt and NF-kappaB signaling pathways, leading to increased levels of Mcl-1, A1, and phosphorylated Bad.
Human CD4+ T cells express TLR5 and its ligand flagellin enhances the suppressive capacity and expression of FOXP3 in CD4+CD25+ T regulatory cells.
Human CD4+ T cells express TLR5 and its ligand flagellin enhances the suppressive capacity and expression of FOXP3 in CD4+CD25+ T regulatory cells.
Identification of hTLR10: a novel human Toll-like receptor preferentially expressed in immune cells.
Identification of hTLR10: a novel human Toll-like receptor preferentially expressed in immune cells.
The human toll signaling pathway: divergence of nuclear factor kappaB and JNK/SAPK activation upstream of tumor necrosis factor receptor-associated factor 6 (TRAF6).
The human toll signaling pathway: divergence of nuclear factor kappaB and JNK/SAPK activation upstream of tumor necrosis factor receptor-associated factor 6 (TRAF6).
Innate immunity: the virtues of a nonclonal system of recognition.
Innate immunity: the virtues of a nonclonal system of recognition.
Hoang TX, Jung JH, Kim JY
BioMed research international 2019;2019:8059312
BioMed research international 2019;2019:8059312
Toll-Like Receptor 1/2 and 5 Ligands Enhance the Expression of Cyclin D1 and D3 and Induce Proliferation in Mantle Cell Lymphoma.
Mastorci K, Muraro E, Pasini E, Furlan C, Sigalotti L, Cinco M, Dolcetti R, Fratta E
PloS one 2016;11(4):e0153823
PloS one 2016;11(4):e0153823
Pseudomonas aeruginosa stimulates tyrosine phosphorylation of and TLR5 association with the MUC1 cytoplasmic tail through EGFR activation.
Kato K, Lillehoj EP, Kim KC
Inflammation research : official journal of the European Histamine Research Society ... [et al.] 2016 Mar;65(3):225-33
Inflammation research : official journal of the European Histamine Research Society ... [et al.] 2016 Mar;65(3):225-33
Vaccination against canine leishmaniosis increases the phagocytic activity, nitric oxide production and expression of cell activation/migration molecules in neutrophils and monocytes.
Moreira ML, Costa-Pereira C, Alves ML, Marteleto BH, Ribeiro VM, Peruhype-Magalhães V, Giunchetti RC, Martins-Filho OA, Araújo MS
Veterinary parasitology 2016 Apr 15;220:33-45
Veterinary parasitology 2016 Apr 15;220:33-45
Inhibition of IRAK1/4 sensitizes T cell acute lymphoblastic leukemia to chemotherapies.
Li Z, Younger K, Gartenhaus R, Joseph AM, Hu F, Baer MR, Brown P, Davila E
The Journal of clinical investigation 2015 Mar 2;125(3):1081-97
The Journal of clinical investigation 2015 Mar 2;125(3):1081-97
Dendritic cells induce Th2-mediated airway inflammatory responses to house dust mite via DNA-dependent protein kinase.
Mishra A, Brown AL, Yao X, Yang S, Park SJ, Liu C, Dagur PK, McCoy JP, Keeran KJ, Nugent GZ, Jeffries KR, Qu X, Yu ZX, Levine SJ, Chung JH
Nature communications 2015 Feb 18;6:6224
Nature communications 2015 Feb 18;6:6224
Flagellin/TLR5 signalling activates renal collecting duct cells and facilitates invasion and cellular translocation of uropathogenic Escherichia coli.
Bens M, Vimont S, Ben Mkaddem S, Chassin C, Goujon JM, Balloy V, Chignard M, Werts C, Vandewalle A
Cellular microbiology 2014 Oct;16(10):1503-17
Cellular microbiology 2014 Oct;16(10):1503-17
Prevention of graft-versus-host disease by adoptive T regulatory therapy is associated with active repression of peripheral blood Toll-like receptor 5 mRNA expression.
Sawitzki B, Brunstein C, Meisel C, Schumann J, Vogt K, Appelt C, Curtsinger JM, Verneris MR, Miller JS, Wagner JE, Blazar BR
Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation 2014 Feb;20(2):173-82
Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation 2014 Feb;20(2):173-82
Characterization of the Toll-like receptor expression profile in human multiple myeloma cells.
Abdi J, Mutis T, Garssen J, Redegeld F
PloS one 2013;8(4):e60671
PloS one 2013;8(4):e60671
Regulation of Toll-like receptor 5 gene expression and function on mucosal dendritic cells.
Feng T, Cong Y, Alexander K, Elson CO
PloS one 2012;7(4):e35918
PloS one 2012;7(4):e35918
The K5 capsule of Escherichia coli strain Nissle 1917 is important in stimulating expression of Toll-like receptor 5, CD14, MyD88, and TRIF together with the induction of interleukin-8 expression via the mitogen-activated protein kinase pathway in epithelial cells.
Hafez M, Hayes K, Goldrick M, Grencis RK, Roberts IS
Infection and immunity 2010 May;78(5):2153-62
Infection and immunity 2010 May;78(5):2153-62
Toll-like receptor triggering in cord blood mesenchymal stem cells.
van den Berk LC, Jansen BJ, Siebers-Vermeulen KG, Netea MG, Latuhihin T, Bergevoet S, Raymakers RA, Kögler G, Figdor CC, Adema GJ, Torensma R
Journal of cellular and molecular medicine 2009 Sep;13(9B):3415-26
Journal of cellular and molecular medicine 2009 Sep;13(9B):3415-26
Toll-like receptor agonists induce inflammation and cell death in a model of head and neck squamous cell carcinomas.
Rydberg C, Månsson A, Uddman R, Riesbeck K, Cardell LO
Immunology 2009 Sep;128(1 Suppl):e600-11
Immunology 2009 Sep;128(1 Suppl):e600-11
Expression patterns and functions of toll-like receptors in mouse sertoli cells.
Wu H, Wang H, Xiong W, Chen S, Tang H, Han D
Endocrinology 2008 Sep;149(9):4402-12
Endocrinology 2008 Sep;149(9):4402-12
Human lymphatic endothelial cells express multiple functional TLRs.
Pegu A, Qin S, Fallert Junecko BA, Nisato RE, Pepper MS, Reinhart TA
Journal of immunology (Baltimore, Md. : 1950) 2008 Mar 1;180(5):3399-405
Journal of immunology (Baltimore, Md. : 1950) 2008 Mar 1;180(5):3399-405
Human lymphatic endothelial cells express multiple functional TLRs.
Pegu A, Qin S, Fallert Junecko BA, Nisato RE, Pepper MS, Reinhart TA
Journal of immunology (Baltimore, Md. : 1950) 2008 Mar 1;180(5):3399-405
Journal of immunology (Baltimore, Md. : 1950) 2008 Mar 1;180(5):3399-405
Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry.
Burgener IA, Jungi TW
Veterinary immunology and immunopathology 2008 Jul 15;124(1-2):184-91
Veterinary immunology and immunopathology 2008 Jul 15;124(1-2):184-91
Intestinal bacteria trigger T cell-independent immunoglobulin A(2) class switching by inducing epithelial-cell secretion of the cytokine APRIL.
He B, Xu W, Santini PA, Polydorides AD, Chiu A, Estrella J, Shan M, Chadburn A, Villanacci V, Plebani A, Knowles DM, Rescigno M, Cerutti A
Immunity 2007 Jun;26(6):812-26
Immunity 2007 Jun;26(6):812-26
Intracellular signaling mechanisms regulating toll-like receptor-mediated activation of eosinophils.
Wong CK, Cheung PF, Ip WK, Lam CW
American journal of respiratory cell and molecular biology 2007 Jul;37(1):85-96
American journal of respiratory cell and molecular biology 2007 Jul;37(1):85-96
Sertoli cells initiate testicular innate immune responses through TLR activation.
Riccioli A, Starace D, Galli R, Fuso A, Scarpa S, Palombi F, De Cesaris P, Ziparo E, Filippini A
Journal of immunology (Baltimore, Md. : 1950) 2006 Nov 15;177(10):7122-30
Journal of immunology (Baltimore, Md. : 1950) 2006 Nov 15;177(10):7122-30
Sertoli cells initiate testicular innate immune responses through TLR activation.
Riccioli A, Starace D, Galli R, Fuso A, Scarpa S, Palombi F, De Cesaris P, Ziparo E, Filippini A
Journal of immunology (Baltimore, Md. : 1950) 2006 Nov 15;177(10):7122-30
Journal of immunology (Baltimore, Md. : 1950) 2006 Nov 15;177(10):7122-30
Modulation of neonatal microbial recognition: TLR-mediated innate immune responses are specifically and differentially modulated by human milk.
LeBouder E, Rey-Nores JE, Raby AC, Affolter M, Vidal K, Thornton CA, Labéta MO
Journal of immunology (Baltimore, Md. : 1950) 2006 Mar 15;176(6):3742-52
Journal of immunology (Baltimore, Md. : 1950) 2006 Mar 15;176(6):3742-52
Modulation of neonatal microbial recognition: TLR-mediated innate immune responses are specifically and differentially modulated by human milk.
LeBouder E, Rey-Nores JE, Raby AC, Affolter M, Vidal K, Thornton CA, Labéta MO
Journal of immunology (Baltimore, Md. : 1950) 2006 Mar 15;176(6):3742-52
Journal of immunology (Baltimore, Md. : 1950) 2006 Mar 15;176(6):3742-52
Toll-like receptors in cellular subsets of human tonsil T cells: altered expression during recurrent tonsillitis.
Mansson A, Adner M, Cardell LO
Respiratory research 2006 Feb 27;7:36
Respiratory research 2006 Feb 27;7:36
Inhibition of neutrophil apoptosis by TLR agonists in whole blood: involvement of the phosphoinositide 3-kinase/Akt and NF-kappaB signaling pathways, leading to increased levels of Mcl-1, A1, and phosphorylated Bad.
François S, El Benna J, Dang PM, Pedruzzi E, Gougerot-Pocidalo MA, Elbim C
Journal of immunology (Baltimore, Md. : 1950) 2005 Mar 15;174(6):3633-42
Journal of immunology (Baltimore, Md. : 1950) 2005 Mar 15;174(6):3633-42
Inhibition of neutrophil apoptosis by TLR agonists in whole blood: involvement of the phosphoinositide 3-kinase/Akt and NF-kappaB signaling pathways, leading to increased levels of Mcl-1, A1, and phosphorylated Bad.
François S, El Benna J, Dang PM, Pedruzzi E, Gougerot-Pocidalo MA, Elbim C
Journal of immunology (Baltimore, Md. : 1950) 2005 Mar 15;174(6):3633-42
Journal of immunology (Baltimore, Md. : 1950) 2005 Mar 15;174(6):3633-42
Human CD4+ T cells express TLR5 and its ligand flagellin enhances the suppressive capacity and expression of FOXP3 in CD4+CD25+ T regulatory cells.
Crellin NK, Garcia RV, Hadisfar O, Allan SE, Steiner TS, Levings MK
Journal of immunology (Baltimore, Md. : 1950) 2005 Dec 15;175(12):8051-9
Journal of immunology (Baltimore, Md. : 1950) 2005 Dec 15;175(12):8051-9
Human CD4+ T cells express TLR5 and its ligand flagellin enhances the suppressive capacity and expression of FOXP3 in CD4+CD25+ T regulatory cells.
Crellin NK, Garcia RV, Hadisfar O, Allan SE, Steiner TS, Levings MK
Journal of immunology (Baltimore, Md. : 1950) 2005 Dec 15;175(12):8051-9
Journal of immunology (Baltimore, Md. : 1950) 2005 Dec 15;175(12):8051-9
Identification of hTLR10: a novel human Toll-like receptor preferentially expressed in immune cells.
Chuang T, Ulevitch RJ
Biochimica et biophysica acta 2001 Mar 19;1518(1-2):157-61
Biochimica et biophysica acta 2001 Mar 19;1518(1-2):157-61
Identification of hTLR10: a novel human Toll-like receptor preferentially expressed in immune cells.
Chuang T, Ulevitch RJ
Biochimica et biophysica acta 2001 Mar 19;1518(1-2):157-61
Biochimica et biophysica acta 2001 Mar 19;1518(1-2):157-61
The human toll signaling pathway: divergence of nuclear factor kappaB and JNK/SAPK activation upstream of tumor necrosis factor receptor-associated factor 6 (TRAF6).
Muzio M, Natoli G, Saccani S, Levrero M, Mantovani A
The Journal of experimental medicine 1998 Jun 15;187(12):2097-101
The Journal of experimental medicine 1998 Jun 15;187(12):2097-101
The human toll signaling pathway: divergence of nuclear factor kappaB and JNK/SAPK activation upstream of tumor necrosis factor receptor-associated factor 6 (TRAF6).
Muzio M, Natoli G, Saccani S, Levrero M, Mantovani A
The Journal of experimental medicine 1998 Jun 15;187(12):2097-101
The Journal of experimental medicine 1998 Jun 15;187(12):2097-101
Innate immunity: the virtues of a nonclonal system of recognition.
Medzhitov R, Janeway CA Jr
Cell 1997 Oct 31;91(3):295-8
Cell 1997 Oct 31;91(3):295-8
Innate immunity: the virtues of a nonclonal system of recognition.
Medzhitov R, Janeway CA Jr
Cell 1997 Oct 31;91(3):295-8
Cell 1997 Oct 31;91(3):295-8
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TLR5 Antibody (85B152.5) [NBP1-97728] - Normal human bronchial epithelial total cell lysate. Image from verified customer review.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TLR5 Antibody (85B152.5) [NBP1-97728] - Intracellular flow analysis of TLR5 in Ramos cells using 1 ug of TLR5 antibody. Shaded histogram represents Ramos cells without antibody; green represents isotype control antibody; red represents TLR5 antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TLR5 Antibody (85B152.5) [NBP1-97728] - Analysis using the FITC conjugate of NBP1-97728. Staining of TLR5 in 10^6 cells using 0.5 ugs of FITC-conjugated antibody: A) Intracellular flow testing of human Ramos cells and B) cell surface flow testing of mouse RAW cells. The shaded (or black) histogram represents cells without antibody, green represents isotype control antibody, and red represents TLR5 antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TLR5 Antibody (85B152.5) [NBP1-97728] - Analysis using the PE conjugate of NBP1-97728. Staining of TLR5 in 10^6 human lymphocytes using 0.5 ugs of PE-conjugated antibody. The shaded histogram represents cells without antibody, green represents isotype control antibody, and red represents TLR5 antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TLR5 Antibody (85B152.5) [NBP1-97728] - Analysis using the PE conjugate of NBP1-97728. Staining of TLR5 in 10^6 RAW cells using 0.5 ugs of PE-conjugated antibody. The black histogram represents cells without antibody, green represents isotype control antibody, and red represents TLR5 antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TLR5 Antibody (85B152.5) [NBP1-97728] - Intracellular flow analysis of TLR5 in 10^6 human lymphocytes using 0.5 ug of NB200-571. The shaded histogram represents cells without antibody, green represents isotype control (BD), and red represents TLR5 antibody.
