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LearnMA5-16233
antibody from Invitrogen Antibodies
Targeting: TLR5
FLJ10052, MGC126430, MGC126431, SLEB1, TIL3
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [6]
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- Product number
- MA5-16233 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TLR5 Monoclonal Antibody (85B152.5)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Suggested positive control: Ramos.
- Reactivity
- Human, Mouse, Canine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 85B152.5
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TLR5 on Lysate from human Ramos (A) and mouse RAW (B) cells using a TLR5 monoclonal antibody (Product # MA5-16233) at 2 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Intracellular flow analysis of TLR5 in Ramos cells using 1 µg of TLR5 monoclonal antibody (Product # MA5-16233). Shaded histogram represents Ramos cells without antibody; green represents isotype control antibody; red represents TLR5 monoclonal antibody (Product # MA5-16233).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of TLR5 in 10^6 cells. Samples were incubated in TLR5 monoclonal antibody (Product # MA5-16233) using a dilution of 0.5 µg. A) Intracellular flow testing of human Ramos cells and B) cell surface flow testing of mouse RAW cells. The shaded (or black) histogram represents cells without antibody, green represents isotype control antibody, and red represents TLR5 antibody (FITC conjugated).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of TLR5 in 10^6 human lymphocytes. Samples were incubated in TLR5 monoclonal antibody (Product # MA5-16233) using a dilution of 0.5 µg. The shaded histogram represents cells without antibody, green represents isotype control antibody, and red represents TLR5 antibody (PE conjugated).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of TLR5 in 10^6 RAW cells. Samples were incubated in TLR5 monoclonal antibody (Product # MA5-16233) using a dilution of 0.5 µg. The black histogram represents cells without antibody, green represents isotype control antibody, and red represents TLR5 antibody (PE conjugated).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of TLR5 in 10^6 human lymphocytes. Samples were incubated in TLR5 monoclonal antibody (Product # MA5-16233) using a dilution of 0.5 µg. The shaded histogram represents cells without antibody, green represents isotype control (BD), and red represents TLR5 antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of TLR5 in Ramos cells. Samples were incubated in TLR5 monoclonal antibody (Product # MA5-16233) using a dilution of 1.0 µg. Shaded histogram represents Ramos cells without antibody; green represents isotype control antibody; red represents TLR5 antibody.
