MA5-23801

antibody from Invitrogen Antibodies

Targeting: LTA LT, TNFB, TNFSF1

ELISA (Recommended by provider)

Blocking/Neutralizing (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-23801

Provider

Invitrogen Antibodies

Product name

Anti-TNF beta Monoclonal Antibody (5807)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

In ELISAs, this antibody shows less than 0.2% cross-reactivity with recombinant mouse (rm) IL-1ra and no cross-reactivity with rhIL-1 alpha, rpIL-1 alpha, rmIL-1 alpha, rrIL-1 alpha, rhIL-1 beta, rpIL-1 beta, rmIL-1 beta, rrIL-1 beta, rhIL-1 RI, or rhIL-1 RII. Reconstitute at 0.5 mg/mL in sterile PBS. Endoxin level is

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

5807

Vial size

1 mg

Concentration

0.5 mg/ml

Storage

-20° C, Avoid Freeze/Thaw Cycles

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Neutralization assay of recombinant human Lymphotoxin-a/TNF-ß induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by recombinant human Lymphotoxin-a/TNF-ß (0.25 ng/mL) is neutralized (green line) by increasing concentrations of mouse Anti-human Lymphotoxin-a/TNF-ß Monoclonal Antibody (Product # MA5-23801). The ND50 is typically 0.03-0.09 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).