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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Flow cytometry [1]
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- Product number
- 17-7327-81 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TNF beta Monoclonal Antibody (359-81-11), APC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The 359-81-11 antibody reacts with Tumor Necrosis Factor-beta (TNF-beta), a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and other target cells.
- Antibody clone number
- 359-81-11
- Concentration
- 0.2 mg/mL
Submitted references Distinct profiles of human B cell effector cytokines: a role in immune regulation?
Tumor eradication by hepatitis B virus X antigen-specific CD8+ T cells in xenografted nude mice.
TCRalphabeta+/CD4+ large granular lymphocytosis: a new clonal T-cell lymphoproliferative disorder.
Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples.
Duddy ME, Alter A, Bar-Or A
Journal of immunology (Baltimore, Md. : 1950) 2004 Mar 15;172(6):3422-7
Journal of immunology (Baltimore, Md. : 1950) 2004 Mar 15;172(6):3422-7
Tumor eradication by hepatitis B virus X antigen-specific CD8+ T cells in xenografted nude mice.
Chun E, Lee J, Cheong HS, Lee KY
Journal of immunology (Baltimore, Md. : 1950) 2003 Feb 1;170(3):1183-90
Journal of immunology (Baltimore, Md. : 1950) 2003 Feb 1;170(3):1183-90
TCRalphabeta+/CD4+ large granular lymphocytosis: a new clonal T-cell lymphoproliferative disorder.
Lima M, Almeida J, Dos Anjos Teixeira M, Alguero Md Mdel C, Santos AH, Balanzategui A, Queirós ML, Bárcena P, Izarra A, Fonseca S, Bueno C, Justiça B, Gonzalez M, San Miguel JF, Orfao A
The American journal of pathology 2003 Aug;163(2):763-71
The American journal of pathology 2003 Aug;163(2):763-71
Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples.
Abrams JS, Roncarolo MG, Yssel H, Andersson U, Gleich GJ, Silver JE
Immunological reviews 1992 Jun;127:5-24
Immunological reviews 1992 Jun;127:5-24
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- CD4-enriched human peripheral blood cells were polarized under Th1 conditions (with Human IL-12 Recombinant Protein (Product # 34-8129-82) and Human IL-2 Recombinant Protein (Product # BMS334) for 10 days. Cells were restimulated with Protein Transport Inhibitor Cocktail (Product #00-4980-03) (left) or with Cell Stimulation Cocktail plus Protein Transport Inhibitors (Product #00-4975-03) (right) for 5 hours. Cells were then stained intracellularly, using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol, with CD4 Monoclonal Antibody, FITC (Product # 11-0049-42) and 0.125 µg TNF beta Monoclonal Antibody, APC. Cells in the lymphocyte gate were used for analysis.
