Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AF-211-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Human Lymphotoxin-alpha/TNF-beta Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human Lymphotoxin-alpha/TNF-beta in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) TNF-alpha , rhsTNF RI and rhsTNF RII is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P01374
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Lymphotoxin-alphabeta heterotrimers are cleaved by metalloproteinases and contribute to synovitis in rheumatoid arthritis.
Recapitulation of B cell differentiation in the central nervous system of patients with multiple sclerosis.
Young J, Yu X, Wolslegel K, Nguyen A, Kung C, Chiang E, Kolumam G, Wei N, Wong WL, DeForge L, Townsend MJ, Grogan JL
Cytokine 2010 Jul;51(1):78-86
Cytokine 2010 Jul;51(1):78-86
Recapitulation of B cell differentiation in the central nervous system of patients with multiple sclerosis.
Corcione A, Casazza S, Ferretti E, Giunti D, Zappia E, Pistorio A, Gambini C, Mancardi GL, Uccelli A, Pistoia V
Proceedings of the National Academy of Sciences of the United States of America 2004 Jul 27;101(30):11064-9
Proceedings of the National Academy of Sciences of the United States of America 2004 Jul 27;101(30):11064-9
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- Cytotoxicity Induced by Lymphotoxin-alpha/TNF-beta and Neutralization by Human Lymphotoxin-alpha/TNF-beta Antibody. Recombinant Human Lymphotoxin-alpha/TNF-beta (Catalog # 211-TB) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Cytotoxicity elicited by Recombinant Human Lymphotoxin-alpha/TNF-beta (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Lymphotoxin-alpha/TNF-beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-211-NA). The ND50 is typically 0.01-0.03 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).