MAB211

antibody from R&D Systems

Targeting: LTA LT, TNFB, TNFSF1

Immunocytochemistry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB211

Provider

R&D Systems

Product name

Human Lymphotoxin-alpha/TNF-beta Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from ascites. Detects human Lymphotoxin-alpha/TNF-beta in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) TNF-alpha is observed. Neutralizes the biological activity of recombinant human TNF-beta . Will not neutralize the biological activity of recombinant human TNF-alpha or recombinant mouse TNF-alpha .

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

P01374


Isotype

IgG

Antibody clone number

5802

Vial size

500 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

True infliximab resistance in rheumatoid arthritis: a role for lymphotoxin alpha?

Buch MH, Conaghan PG, Quinn MA, Bingham SJ, Veale D, Emery P
Annals of the rheumatic diseases 2004 Oct;63(10):1344-6

Immunocytochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Lymphotoxin-alpha/TNF-beta in Human PBMCs. Lymphotoxin-alpha/TNF-beta was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with LPS and monensin using Mouse Anti-Human Lymphotoxin-alpha/TNF-beta Monoclonal Antibody (Catalog # MAB211) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Blocking/Neutralizing

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Cytotoxicity Induced by Lymphotoxin-alpha/TNF-beta and Neutral-ization by Human Lymphotoxin-alpha/TNF-beta Antibody. Recombinant Human Lymphotoxin-alpha/TNF-beta (Catalog # 211-TB) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Cytotoxicity elicited by Recombinant Human Lymphotoxin-alpha/TNF-beta (50 pg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Lymphotoxin-alpha/TNF-beta Monoclonal Antibody (Catalog # MAB211). The ND₅₀ is typically 75-600 ng/mL in the presence of the metabolic inhibitor actinomycin D.