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- Antigen structure
- References [3]
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- Product number
- M3TNFABI - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TNF alpha Monoclonal Antibody (E10041), Biotin
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- M3TNFABI detects TNF Alpha which has a predicted molecular weight of approximately 26 kDa. M3TNFABI targets TNF Alpha in ELISA and Western blot applications and shows reactivity with Canine, Feline, Human, Non-human primate, and Porcine samples. The MTNFAI antibody (clone E10043) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody M3TNFABI (biotinylated conjugate of clone E10041).
- Conjugate
- Biotin
- Antibody clone number
- E10041
- Concentration
- 0.1 mg/mL
Submitted references Imaging of Protein Secretion from a Single Cell Using Plasmonic Substrates.
Enhanced interleukin-12 and CD40 ligand activities but reduced Staphylococcus aureus Cowan 1-induced responses suggest a generalized and progressively impaired type 1 cytokine pattern for human schistosomiasis.
Enhanced interleukin-12 and CD40 ligand activities but reduced Staphylococcus aureus Cowan 1-induced responses suggest a generalized and progressively impaired type 1 cytokine pattern for human schistosomiasis.
Szmacinski H, Toshchakov V, Piao W, Lakowicz JR
BioNanoScience 2013 Mar 1;3(1):30-36
BioNanoScience 2013 Mar 1;3(1):30-36
Enhanced interleukin-12 and CD40 ligand activities but reduced Staphylococcus aureus Cowan 1-induced responses suggest a generalized and progressively impaired type 1 cytokine pattern for human schistosomiasis.
Montenegro SM, Abath FG, Domingues AL, Melo WG, Morais CN, Coutinho EM, Mahanty S, Wynn TA
Infection and immunity 2002 Nov;70(11):5903-12
Infection and immunity 2002 Nov;70(11):5903-12
Enhanced interleukin-12 and CD40 ligand activities but reduced Staphylococcus aureus Cowan 1-induced responses suggest a generalized and progressively impaired type 1 cytokine pattern for human schistosomiasis.
Montenegro SM, Abath FG, Domingues AL, Melo WG, Morais CN, Coutinho EM, Mahanty S, Wynn TA
Infection and immunity 2002 Nov;70(11):5903-12
Infection and immunity 2002 Nov;70(11):5903-12
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- NULL
- Conjugate
- Biotin
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Direct ELISA analysis of TNF alpha was performed by coating wells of a 96-well plate with 100 µL per well of human recombinant protein TNF alpha (Product # RTNFAI) diluted in carbonate/bicarbonate buffer (Product # 28382) at a concentration of 1 µg/mL overnight at 4C. Wells of the plate were washed, blocked with starting blocking buffer (Product # 37538), and incubated with 100 µL per well of a biotin conjugated mouse anti-TNF alpha monoclonal antibody (Product # M3TNFABI) at a dilution of 1:25, 1:50, 1:100, 1:200, 1:400, 1:800, 1:1600 and 1:3200 for 90 minutes at 37C. The plate was washed, then incubated with 100 µL per well of a pre-diluted High Sensitivity Streptavidin-HRP antibody (Product # 21134) at a dilution of 1:50 for 90 minutes at 37C. Detection was performed using 1-Step Ultra TMB substrate (Product # 34028) for 5-10 minutes at room temperature in the dark. The reaction was stopped with Stop solution (Product # N600), and absorbances were read on a spectrophotometer at 450-550 nm.
- Conjugate
- Biotin
