MTNFAI

antibody from Invitrogen Antibodies

Targeting: TNF DIF, TNF-alpha, TNFA, TNFSF2

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MTNFAI

Provider

Invitrogen Antibodies

Product name

TNF alpha Monoclonal Antibody (E10043)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

MTNFAI targets TNF Alpha in ELISA, and Western blot applications and shows reactivity with Human samples. The MTNFAI antibody (clone E10043) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody M3TNFABI (biotinylated conjugate of clone E10041).

Antibody clone number

E10043

Concentration

0.5 mg/mL

References

Submitted references

Alzheimer's associated β-amyloid protein inhibits influenza A virus and modulates viral interactions with phagocytes.

White MR, Kandel R, Tripathi S, Condon D, Qi L, Taubenberger J, Hartshorn KL
PloS one 2014;9(7):e101364

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Imaging of Protein Secretion from a Single Cell Using Plasmonic Substrates.

Szmacinski H, Toshchakov V, Piao W, Lakowicz JR
BioNanoScience 2013 Mar 1;3(1):30-36

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Hapivirins and diprovirins: novel θ-defensin analogs with potent activity against influenza A virus.

Doss M, Ruchala P, Tecle T, Gantz D, Verma A, Hartshorn A, Crouch EC, Luong H, Micewicz ED, Lehrer RI, Hartshorn KL
Journal of immunology (Baltimore, Md. : 1950) 2012 Mar 15;188(6):2759-68

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Time-Resolved Fluorometric Method for One-Step Immunoassays Using Plasmonic Nanostructures.

Szmacinski H, Murtaza Z, Lakowicz JR
The journal of physical chemistry. C, Nanomaterials and interfaces 2010 Apr 29;114(16):7236-7241

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Direct ELISA analysis of TNF alpha was performed by coating wells of a 96-well plate with 100 µL per well of human recombinant protein TNF alpha (Product # RTNFAI) diluted in carbonate/bicarbonate buffer (Product # 28382) at a concentration of 1 µg/mL overnight at 4C. Wells of the plate were washed, blocked with starting blocking buffer (Product # 37538), and incubated with 100 µL per well of a mouse anti-TNF alpha monoclonal antibody (Product # MTNFAI) at a dilution of 1:25, 1:50, 1:100, 1:200, 1:400, 1:800, 1:1600 and 1:3200 for 90 minutes at 37C. The plate was washed, then incubated with 100 µL per well of a HRP-conjugated goat anti-mouse IgG secondary antibody (Product # 62-6520) at a dilution of 1:2000 for 90 minutes at 37C. Detection was performed using 1-Step Ultra TMB substrate (Product # 34028) for 5-10 minutes at room temperature in the dark. The reaction was stopped with Stop solution (Product # N600), and absorbances were read on a spectrophotometer at 450-550 nm.