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ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- GTX48753 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX48753, RRID:AB_11168213
- Product name
- TNF alpha antibody
- Antibody type
- Polyclonal
- Reactivity
- Porcine
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot using GeneTex's protein-A purified anti-swine TNFa antibody shows detection of recombinant Swine TNFa at about 16.9 kDa raised in yeast. The identity of the faint band at 135 kDa is not known. The protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1:10), and probed with 1 µg/mL primary antibody overnight at 4°C. After washing, membrane was probed with IRDye800 Conjugated Goat Anti-Rabbit IgG for 45 min at room temperature.
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot using GeneTex's protein-A purified anti-pig TNFa antibody shows detection of recombinant pig TNFa at about 16.9 kDa raised in yeast. The identity of the faint band at 135 kDa is not known. The protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA , and probed with 1 ?g/mL primary antibody overnight at 4¢XC. After washing, membrane was probed with IRDye800 Conjugated Goat Anti-Rabbit IgG for 45 min at room temperature.
