AF1507

antibody from Novus Biologicals

Targeting: TNF DIF, TNF-alpha, TNFA, TNFSF2

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

AF1507

Provider

Novus Biologicals

Product name

Goat Polyclonal TNF-alpha Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects canine TNF-alpha direct ELISAs and Western blots. In direct ELISAs, approximately 70% cross-reactivity with recombinant human TNF-alpha , 50% cross-reactivity with recombinant rhesus macaque TNF-alpha , 35% cross-reactivity with recombinant equine TNF-alpha , recombinant cotton rat TNF-alpha and recombinant rat TNF-alpha , 25% cross-reactivity with recombinant mouse TNF-alpha , and 10% cross-reactivity with recombinant porcine TNF-alpha is observed.

Reactivity

Canine

Host

Goat

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Blocking/Neutralizing

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Cytotoxicity Induced by TNF-alpha and Neutralization by Canine TNF-alpha Antibody. Recombinant Canine TNF-alpha (Catalog # 1507-CT) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Cytotoxicity elicited by Recombinant Canine TNF-alpha (100 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Canine TNF-alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1507). The ND₅₀ is typically 0.4-1.6 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).