Explore
Validate
Learn
Other assayAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 701264 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TNF alpha Recombinant Rabbit Monoclonal Antibody (3H15L1)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- P300A was successfully used to detect human TNF alpha in ELISA application.
- Antibody clone number
- 3H15L1
- Concentration
- 0.5 mg/mL
Submitted references Retinol dehydrogenase 13 protects the mouse retina from acute light damage.
Wang H, Cui X, Gu Q, Chen Y, Zhou J, Kuang Y, Wang Z, Xu X
Molecular vision 2012;18:1021-30
Molecular vision 2012;18:1021-30
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Direct ELISA analysis of TNF alpha was performed by coating wells of a 96-well plate with 100 µL per well of human recombinant protein TNF alpha (Product # RTNFAI) diluted in carbonate/bicarbonate buffer (Product # 28382) at a concentration of 1 µg/mL overnight at 4C. Wells of the plate were washed, blocked with starting blocking buffer (Product # 37538), and incubated with 100 µL per well of a rabbit anti-TNF alpha monoclonal antibody (Product # M302) at a dilution of 1:25, 1:50, 1:100, 1:200, 1:400, 1:800, 1:1600 and 1:3200 for 90 minutes at 37C. The plate was washed, then incubated with 100 µL per well of a HRP-conjugated goat anti-rabbit IgG secondary antibody (Product # 65-6120) at a dilution of 1:2000 for 90 minutes at 37C. Detection was performed using 1-Step Ultra TMB substrate (Product # 34028) for 5-10 minutes at room temperature in the dark. The reaction was stopped with Stop solution (Product # N600), and absorbances were read on a spectrophotometer at 450-550 nm.
