Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB10701 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal TNF-alpha Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects rhesus macaque TNF-alpha in direct ELISAs and Western blots. In direct ELISAs, 10-25% cross-reactivity with recombinant human (rh) TNF-alpha and recombinant porcine TNF-alpha is observed and no cross-reactivity with rhEDA-A2, recombinant mouse (rm) EDA, rhAPRIL, rhBAFF, rhFas L, rhGITR L, rhLIGHT, rhLymphotoxin alpha 1 beta 2, rhLymphotoxin alpha 2 beta 1, rhOX40 L, recombinant cotton rat TNF-alpha , rmTNF-alpha , recombinant rat TNF-alpha , rhTRAIL, rhTRANCE, and rhTWEAK is observed.
- Host
- Rat
- Isotype
- IgG
- Vial size
- 500 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Cytotoxicity Induced by TNF-alpha and Neutralization by Rhesus Macaque TNF-alpha Antibody. Recombinant Rhesus Macaque TNF-alpha (Catalog # 1070-RM) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Cytotoxicity elicited by Recombinant Rhesus Macaque TNF-alpha (1 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Rhesus Macaque TNF-alpha Monoclonal Antibody (Catalog # MAB10701). The ND50 is typically 0.2-0.8 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).