FAB210F-050

antibody from Novus Biologicals

Targeting: TNF DIF, TNF-alpha, TNFA, TNFSF2

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

FAB210F-050

Provider

Novus Biologicals

Product name

Mouse Monoclonal TNF-alpha Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from ascites. Detects cell surface expressed TNF-alpha (membrane and receptor bound forms) by flow cytometry. Detects human TNF-alpha in direct ELISAs and Western blots. In direct ELISAs, approximately 25-50% cross-reactivity with recombinant porcine TNF-alpha and recombinant rhesus macaque TNF-alpha is observed but no cross-reactivity with recombinant cotton rat TNF-alpha , recombinant rat TNF-alpha , recombinant human (rh) LT alpha 1/beta 2, rhLT alpha 2/beta 1, rhAPRIL, rhBAFF, rhEDA-A2, recombinant mouse EDA, rhFas Ligand, rhLIGHT, rhOX40 Ligand, rhTRAIL, rhTRANCE, rhTWEAK, or rhVEGI is observed.

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

50 Tests

Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of TNF-alpha in Human Peripheral Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human TNF-alpha Membrane Form Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB210F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.