BAF2279

antibody from R&D Systems

Targeting: TNF DIF, TNF-alpha, TNFA, TNFSF2

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

BAF2279

Provider

R&D Systems

Product name

Bovine TNF-alpha Biotinylated Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects TNF-alpha in ELISAs and Western blots. In sandwich immunoassays, less than 0.3% cross-reactivity with recombinant human TNF-alpha , recombinant mouse TNF-alpha , recombinant rat TNF-alpha , recombinant feline TNF-alpha , recombinant equine TNF-alpha , recombinant porcine TNF-alpha , recombinant cotton rat TNF-alpha , and rhesus macaque TNF-alpha is observed.

Reactivity

Bovine

Host

Goat

Conjugate

Biotin

Antigen sequence

Q06599


Isotype

IgG

Vial size

50 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Immunocytochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

TNF-alpha in Bovine PBMCs. TNF-alpha was detected in immersion fixed bovine peripheral blood mononuclear cells (PBMCs) using Goat Anti-Bovine TNF-alpha Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2279) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.