Antibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB510-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Rat TNF-alpha Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects rat TNF-alpha in ELISAs and Western blots. In ELISAs, this antibody shows less than 3% cross-reactivity with recombinant mouse (rm) TNF-alpha and less than 0.2% cross-reactivity with rhTNF-alpha , rpTNF-alpha , and rhTNF-beta . In Western blots, this antibody shows 100% cross-reactivity with rmTNF-alpha and no cross-reactivity with rfeTNF-alpha .
- Reactivity
- Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P16599
- Isotype
- IgG
- Antibody clone number
- 45418
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Interleukin-4 and Interleukin-13 Exacerbate Neurotoxicity of Prothrombin Kringle-2 in Cortex In Vivo via Oxidative Stress.
Ulva lactuca polysaccharides prevent Wistar rat breast carcinogenesis through the augmentation of apoptosis, enhancement of antioxidant defense system, and suppression of inflammation.
Transport patterns of anti-TNF-α in burn wounds: Therapeutic implications of hyaluronic acid conjugation.
Beneficial Effect of Moderate Exercise in Kidney of Rat after Chronic Consumption of Cola Drinks.
Urban traffic-derived nanoparticulate matter reduces neurite outgrowth via TNFα in vitro.
Neuronal synapse formation induced by microglia and interleukin 10.
In vivo modulation of LPS-induced alterations in brain and peripheral cytokines and HPA axis activity by cannabinoids.
Oxidative mitochondrial DNA damage and deletion in hepatocytes of rejecting liver allografts in rats: role of TNF-alpha.
Jeong JY, Chung YC, Jin BK
International journal of molecular sciences 2019 Apr 19;20(8)
International journal of molecular sciences 2019 Apr 19;20(8)
Ulva lactuca polysaccharides prevent Wistar rat breast carcinogenesis through the augmentation of apoptosis, enhancement of antioxidant defense system, and suppression of inflammation.
Abd-Ellatef GF, Ahmed OM, Abdel-Reheim ES, Abdel-Hamid AZ
Breast cancer (Dove Medical Press) 2017;9:67-83
Breast cancer (Dove Medical Press) 2017;9:67-83
Transport patterns of anti-TNF-α in burn wounds: Therapeutic implications of hyaluronic acid conjugation.
Friedrich EE, Washburn NR
Biomaterials 2017 Jan;114:10-22
Biomaterials 2017 Jan;114:10-22
Beneficial Effect of Moderate Exercise in Kidney of Rat after Chronic Consumption of Cola Drinks.
Cao G, González J, Müller A, Ottaviano G, Ambrosio G, Toblli JE, Milei J
PloS one 2016;11(3):e0152461
PloS one 2016;11(3):e0152461
Urban traffic-derived nanoparticulate matter reduces neurite outgrowth via TNFα in vitro.
Cheng H, Davis DA, Hasheminassab S, Sioutas C, Morgan TE, Finch CE
Journal of neuroinflammation 2016 Jan 26;13:19
Journal of neuroinflammation 2016 Jan 26;13:19
Neuronal synapse formation induced by microglia and interleukin 10.
Lim SH, Park E, You B, Jung Y, Park AR, Park SG, Lee JR
PloS one 2013;8(11):e81218
PloS one 2013;8(11):e81218
In vivo modulation of LPS-induced alterations in brain and peripheral cytokines and HPA axis activity by cannabinoids.
Roche M, Diamond M, Kelly JP, Finn DP
Journal of neuroimmunology 2006 Dec;181(1-2):57-67
Journal of neuroimmunology 2006 Dec;181(1-2):57-67
Oxidative mitochondrial DNA damage and deletion in hepatocytes of rejecting liver allografts in rats: role of TNF-alpha.
Nagakawa Y, Williams GM, Zheng Q, Tsuchida A, Aoki T, Montgomery RA, Klein AS, Sun Z
Hepatology (Baltimore, Md.) 2005 Jul;42(1):208-15
Hepatology (Baltimore, Md.) 2005 Jul;42(1):208-15
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cytotoxicity Induced by TNF-alpha and Neutralization by Rat TNF-alpha Antibody. Recombinant Rat TNF-alpha (Catalog # 510-RT) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Cytotoxicity elicited by Recombinant Rat TNF-alpha (0.025 ng/mL) is neutralized (green line) by increasing concentrations of Rat TNF-alpha Monoclonal Antibody (Catalog # MAB510). The ND50 is typically 10-40 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).