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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [12]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB210-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human TNF-alpha Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human TNF-alpha in direct ELISAs. In direct ELISAs, approximately 50% cross-reactivity with recombinant rhesus macaque TNF-alpha is observed and no cross-reactivity with recombinant cotton rat, mouse, rat, or porcine TNF-alpha is observed. It shows no cross-reactivity with recombinant human (rh) APRIL, rhBAFF, rhEDA-A2, rhFas Ligand, rhGITR Ligand, rhLIGHT, rhOX40 Ligand, rhRANK Ligand, rhTNF-beta , rhTRAIL, rhTRANCE, rhTWEAK, or rhVEGI.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P01375- Isotype
- IgG
- Antibody clone number
- 1825
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Innate activation of human primary epithelial cells broadens the host response to Mycobacterium tuberculosis in the airways.
Antigen delivery to dendritic cells shapes human CD4+ and CD8+ T cell memory responses to Staphylococcus aureus.
Activin A as a mediator of NK-dendritic cell functional interactions.
Monocytes induce STAT3 activation in human mesenchymal stem cells to promote osteoblast formation.
IL-26 is overexpressed in rheumatoid arthritis and induces proinflammatory cytokine production and Th17 cell generation.
KiSS1 metastasis suppressor gene product induces suppression of tyrosine kinase receptor signaling to Akt, tumor necrosis factor family ligand expression, and apoptosis.
Inflammatory cytokines stimulate the adhesion of colon carcinoma cells to mesothelial monolayers.
Peroxisome proliferator-activated receptor-gamma agonists inhibit respiratory syncytial virus-induced expression of intercellular adhesion molecule-1 in human lung epithelial cells.
Evidence for downregulation of erythropoietin receptor in bone marrow erythroid cells of patients with chronic idiopathic neutropenia.
Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription.
Effect of cognate human CD4+ T cell and endothelial cell interactions upon chemokine production.
A cytokine-to-chemokine axis between T lymphocytes and keratinocytes can favor Th1 cell accumulation in chronic inflammatory skin diseases.
Reuschl AK, Edwards MR, Parker R, Connell DW, Hoang L, Halliday A, Jarvis H, Siddiqui N, Wright C, Bremang S, Newton SM, Beverley P, Shattock RJ, Kon OM, Lalvani A
PLoS pathogens 2017 Sep;13(9):e1006577
PLoS pathogens 2017 Sep;13(9):e1006577
Antigen delivery to dendritic cells shapes human CD4+ and CD8+ T cell memory responses to Staphylococcus aureus.
Uebele J, Stein C, Nguyen MT, Schneider A, Kleinert F, Tichá O, Bierbaum G, Götz F, Bekeredjian-Ding I
PLoS pathogens 2017 May;13(5):e1006387
PLoS pathogens 2017 May;13(5):e1006387
Activin A as a mediator of NK-dendritic cell functional interactions.
Seeger P, Bosisio D, Parolini S, Badolato R, Gismondi A, Santoni A, Sozzani S
Journal of immunology (Baltimore, Md. : 1950) 2014 Feb 1;192(3):1241-8
Journal of immunology (Baltimore, Md. : 1950) 2014 Feb 1;192(3):1241-8
Monocytes induce STAT3 activation in human mesenchymal stem cells to promote osteoblast formation.
Nicolaidou V, Wong MM, Redpath AN, Ersek A, Baban DF, Williams LM, Cope AP, Horwood NJ
PloS one 2012;7(7):e39871
PloS one 2012;7(7):e39871
IL-26 is overexpressed in rheumatoid arthritis and induces proinflammatory cytokine production and Th17 cell generation.
Corvaisier M, Delneste Y, Jeanvoine H, Preisser L, Blanchard S, Garo E, Hoppe E, Barré B, Audran M, Bouvard B, Saint-André JP, Jeannin P
PLoS biology 2012;10(9):e1001395
PLoS biology 2012;10(9):e1001395
KiSS1 metastasis suppressor gene product induces suppression of tyrosine kinase receptor signaling to Akt, tumor necrosis factor family ligand expression, and apoptosis.
Navenot JM, Fujii N, Peiper SC
Molecular pharmacology 2009 May;75(5):1074-83
Molecular pharmacology 2009 May;75(5):1074-83
Inflammatory cytokines stimulate the adhesion of colon carcinoma cells to mesothelial monolayers.
van Grevenstein WM, Hofland LJ, van Rossen ME, van Koetsveld PM, Jeekel J, van Eijck CH
Digestive diseases and sciences 2007 Oct;52(10):2775-83
Digestive diseases and sciences 2007 Oct;52(10):2775-83
Peroxisome proliferator-activated receptor-gamma agonists inhibit respiratory syncytial virus-induced expression of intercellular adhesion molecule-1 in human lung epithelial cells.
Arnold R, Neumann M, König W
Immunology 2007 May;121(1):71-81
Immunology 2007 May;121(1):71-81
Evidence for downregulation of erythropoietin receptor in bone marrow erythroid cells of patients with chronic idiopathic neutropenia.
Pontikoglou C, Liapakis G, Pyrovolaki K, Papadakis M, Bux J, Eliopoulos GD, Papadaki HA
Experimental hematology 2006 Oct;34(10):1312-22
Experimental hematology 2006 Oct;34(10):1312-22
Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription.
Ebert EC, Mehta V
Cellular immunology 2006 May;241(1):7-13
Cellular immunology 2006 May;241(1):7-13
Effect of cognate human CD4+ T cell and endothelial cell interactions upon chemokine production.
Tay SS, McCormack A, Rose ML
Transplantation 2004 Oct 15;78(7):987-94
Transplantation 2004 Oct 15;78(7):987-94
A cytokine-to-chemokine axis between T lymphocytes and keratinocytes can favor Th1 cell accumulation in chronic inflammatory skin diseases.
Albanesi C, Scarponi C, Sebastiani S, Cavani A, Federici M, Sozzani S, Girolomoni G
Journal of leukocyte biology 2001 Oct;70(4):617-23
Journal of leukocyte biology 2001 Oct;70(4):617-23
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cytotoxicity Induced by TNF-alpha and Neutralization by Human TNF-alpha Antibody. Recombinant Human TNF-alpha (Catalog # 210-TA) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Human TNF-alpha (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human TNF-alpha Monoclonal Antibody (Catalog # MAB210). The ND50 is typically 0.01-0.06 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).
