Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- FAB210F-050 - Provider product page
- Provider
- R&D Systems
- Product name
- Human TNF-alpha Membrane Form Fluorescein-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects cell surface expressed TNF-alpha (membrane and receptor bound forms) by flow cytometry. Detects human TNF-alpha in direct ELISAs and Western blots. In direct ELISAs, approximately 25-50% cross-reactivity with recombinant porcine TNF-alpha and recombinant rhesus macaque TNF-alpha is observed but no cross-reactivity with recombinant cotton rat TNF-alpha , recombinant rat TNF-alpha , recombinant human (rh) LT alpha 1/beta 2, rhLT alpha 2/beta 1, rhAPRIL, rhBAFF, rhEDA-A2, recombinant mouse EDA, rhFas Ligand, rhLIGHT, rhOX40 Ligand, rhTRAIL, rhTRANCE, rhTWEAK, or rhVEGI is observed.
- Reactivity
- Human
- Host
- Mouse
- Antigen sequence
P01375
- Isotype
- IgG
- Antibody clone number
- 6401
- Vial size
- 50 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references Unlike for human monocytes after LPS activation, release of TNF-α by THP-1 cells is produced by a TACE catalytically different from constitutive TACE.
Simultaneous induction of apoptotic and survival signaling pathways in macrophage-like THP-1 cells by Shiga toxin 1.
Regulation of peritoneal and systemic neutrophil-derived tumor necrosis factor-alpha release in patients with severe peritonitis: role of tumor necrosis factor-alpha converting enzyme cleavage.
Tumour necrosis factor-alpha transcription in transferrin-stimulated human blood mononuclear cells: is transferrin receptor involved in the signalling mechanism?
Moreira-Tabaka H, Peluso J, Vonesch JL, Hentsch D, Kessler P, Reimund JM, Dumont S, Muller CD
PloS one 2012;7(3):e34184
PloS one 2012;7(3):e34184
Simultaneous induction of apoptotic and survival signaling pathways in macrophage-like THP-1 cells by Shiga toxin 1.
Lee SY, Cherla RP, Tesh VL
Infection and immunity 2007 Mar;75(3):1291-302
Infection and immunity 2007 Mar;75(3):1291-302
Regulation of peritoneal and systemic neutrophil-derived tumor necrosis factor-alpha release in patients with severe peritonitis: role of tumor necrosis factor-alpha converting enzyme cleavage.
Kermarrec N, Selloum S, Plantefeve G, Chosidow D, Paoletti X, Lopez A, Mantz J, Desmonts JM, Gougerot-Pocidalo MA, Chollet-Martin S
Critical care medicine 2005 Jun;33(6):1359-64
Critical care medicine 2005 Jun;33(6):1359-64
Tumour necrosis factor-alpha transcription in transferrin-stimulated human blood mononuclear cells: is transferrin receptor involved in the signalling mechanism?
Lopez M, Rios E, Schlesinger L, Olivares M, Nunez MT, Munoz C
British journal of haematology 2003 Mar;120(5):829-35
British journal of haematology 2003 Mar;120(5):829-35
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Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- Detection of TNF-alpha in Human Peripheral Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human TNF-alpha Membrane Form Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB210F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.