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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB10111 - Provider product page
- Provider
- R&D Systems
- Product name
- Cotton Rat TNF-alpha Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects cotton rat TNF-alpha in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) TNF-alpha is observed and no cross-reactivity with rhAPRIL, rhBAFF, rhEDA-A2, recombinant mouse (rm) EDA, rhFas Ligand, rhGITR Ligand, rhLIGHT, rhOX40 Ligand, rmTNF-alpha , recombinant porcine TNF-alpha , recombinant rat TNF-alpha , recombinant rhesus macaque TNF-alpha , rhTRAIL, rmTRAIL, rhTRANCE, rhTWEAK, or rhVEGI is observed.
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
AAL18818- Isotype
- IgG
- Antibody clone number
- 159813
- Vial size
- 500 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Cytotoxicity Induced by TNF-alpha and Neutralization by Cotton Rat TNF-alpha Antibody. Recombinant Cotton Rat TNF-alpha (Catalog # 1011-CR) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Cytotoxicity elicited by Recombinant Cotton Rat TNF-alpha (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Cotton Rat TNF-alpha Monoclonal Antibody (Catalog # MAB10111). The ND50 is typically 0.04-0.2 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).
