Antibody data
- Antibody Data
- Antigen structure
- References [23]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- AF-510-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Rat TNF-alpha Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects rat TNF-alpha in direct ELISAs and Western blots. In Western blots, approximately 25% cross-reactivity with recombinant porcine TNF-alpha is observed and less than 1% cross-reactivity with recombinant human (rh) TNF-beta , rhTNF sRI, and rhTNF sRII is observed. In direct ELISAs, approximately 40% cross-reactivity with recombinant canine TNF-alpha , recombinant feline TNF-alpha , and recombinant rhesus macaque TNF-alpha is observed. Neutralizes the biological activity of rat TNF-alpha . This antibody will also neutralize the biological activity of murine TNF-alpha , but weak neutralization of rhTNF-alpha and no neutralization of rhTNF-beta was observed.
- Reactivity
- Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P16599
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Blocking p38 Signaling Reduces the Activation of Pro-inflammatory Cytokines and the Phosphorylation of p38 in the Habenula and Reverses Depressive-Like Behaviors Induced by Neuroinflammation.
Baicalein attenuates neuropathic pain and improves sciatic nerve function recovery in rats with partial sciatic nerve transection.
Intrinsic Inflammation Is a Potential Anti-Epileptogenic Target in the Organotypic Hippocampal Slice Model.
Aortic dysfunction in metabolic syndrome mediated by perivascular adipose tissue TNFα- and NOX2-dependent pathway.
Effects of TNF-α on penile structure alteration in rats with hyperprolactinemia.
Regulation of kindling epileptogenesis by hippocampal Toll-like receptors 2.
Prolonged neuroinflammation after lipopolysaccharide exposure in aged rats.
Time-dependent effects of training on cardiovascular control in spontaneously hypertensive rats: role for brain oxidative stress and inflammation and baroreflex sensitivity.
Microglial cells contribute to endogenous brain defenses after acute neonatal focal stroke.
Distribution and tumor necrosis factor-alpha isoform binding specificity of locally administered etanercept into injured and uninjured rat sciatic nerve.
Adenovirus-mediated kallistatin gene transfer ameliorates disease progression in a rat model of osteoarthritis induced by anterior cruciate ligament transection.
Mechanisms underlying the nociceptive responses induced by platelet-activating factor (PAF) in the rat paw.
Mechanisms underlying the nociceptive responses induced by platelet-activating factor (PAF) in the rat paw.
Tumor necrosis factor alpha: activity dependent expression and promotion of cortical column sleep in rats.
Supraspinal glial-neuronal interactions contribute to descending pain facilitation.
Non-steroidal anti-inflammatory drug-induced small intestinal damage is Toll-like receptor 4 dependent.
Deleterious role of TNF-alpha in retinal ischemia-reperfusion injury.
Wnt-induced secreted protein-1 is a prohypertrophic and profibrotic growth factor.
TNFalpha and TNF receptor 1 expression in the mixed neuronal-glial cultures of hippocampal dentate gyrus exposed to glutamate or trimethyltin.
Thrombin induces neurodegeneration and microglial activation in the cortex in vivo and in vitro: proteolytic and non-proteolytic actions.
TNFalpha-induced AMPA-receptor trafficking in CNS neurons; relevance to excitotoxicity?
Differential cytokine response in interstitial fluid in skin and serum during experimental inflammation in rats.
Glucocorticoids play a fundamental role in protecting the brain during innate immune response.
Zhao YW, Pan YQ, Tang MM, Lin WJ
Frontiers in pharmacology 2018;9:511
Frontiers in pharmacology 2018;9:511
Baicalein attenuates neuropathic pain and improves sciatic nerve function recovery in rats with partial sciatic nerve transection.
Lai HC, Lu CH, Wong CS, Lin BF, Chan SM, Kuo CY, Wu ZF
Journal of the Chinese Medical Association : JCMA 2018 Nov;81(11):955-963
Journal of the Chinese Medical Association : JCMA 2018 Nov;81(11):955-963
Intrinsic Inflammation Is a Potential Anti-Epileptogenic Target in the Organotypic Hippocampal Slice Model.
Chong SA, Balosso S, Vandenplas C, Szczesny G, Hanon E, Claes K, Van Damme X, Danis B, Van Eyll J, Wolff C, Vezzani A, Kaminski RM, Niespodziany I
Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics 2018 Apr;15(2):470-488
Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics 2018 Apr;15(2):470-488
Aortic dysfunction in metabolic syndrome mediated by perivascular adipose tissue TNFα- and NOX2-dependent pathway.
DeVallance E, Branyan KW, Lemaster K, Olfert IM, Smith DM, Pistilli EE, Frisbee JC, Chantler PD
Experimental physiology 2018 Apr 1;103(4):590-603
Experimental physiology 2018 Apr 1;103(4):590-603
Effects of TNF-α on penile structure alteration in rats with hyperprolactinemia.
Wang ZL, Yang LY, Chen HH, Lin HH, Tsai YT, Huang WJ
PloS one 2017;12(8):e0181952
PloS one 2017;12(8):e0181952
Regulation of kindling epileptogenesis by hippocampal Toll-like receptors 2.
Medel-Matus JS, Reynolds A, Shin D, Sankar R, Mazarati A
Epilepsia 2017 Aug;58(8):e122-e126
Epilepsia 2017 Aug;58(8):e122-e126
Prolonged neuroinflammation after lipopolysaccharide exposure in aged rats.
Fu HQ, Yang T, Xiao W, Fan L, Wu Y, Terrando N, Wang TL
PloS one 2014;9(8):e106331
PloS one 2014;9(8):e106331
Time-dependent effects of training on cardiovascular control in spontaneously hypertensive rats: role for brain oxidative stress and inflammation and baroreflex sensitivity.
Masson GS, Costa TS, Yshii L, Fernandes DC, Soares PP, Laurindo FR, Scavone C, Michelini LC
PloS one 2014;9(5):e94927
PloS one 2014;9(5):e94927
Microglial cells contribute to endogenous brain defenses after acute neonatal focal stroke.
Faustino JV, Wang X, Johnson CE, Klibanov A, Derugin N, Wendland MF, Vexler ZS
The Journal of neuroscience : the official journal of the Society for Neuroscience 2011 Sep 7;31(36):12992-3001
The Journal of neuroscience : the official journal of the Society for Neuroscience 2011 Sep 7;31(36):12992-3001
Distribution and tumor necrosis factor-alpha isoform binding specificity of locally administered etanercept into injured and uninjured rat sciatic nerve.
Kato K, Kikuchi S, Shubayev VI, Myers RR
Neuroscience 2009 May 5;160(2):492-500
Neuroscience 2009 May 5;160(2):492-500
Adenovirus-mediated kallistatin gene transfer ameliorates disease progression in a rat model of osteoarthritis induced by anterior cruciate ligament transection.
Hsieh JL, Shen PC, Shiau AL, Jou IM, Lee CH, Teo ML, Wang CR, Chao J, Chao L, Wu CL
Human gene therapy 2009 Feb;20(2):147-58
Human gene therapy 2009 Feb;20(2):147-58
Mechanisms underlying the nociceptive responses induced by platelet-activating factor (PAF) in the rat paw.
Marotta DM, Costa R, Motta EM, Fernandes ES, Medeiros R, Quintão NL, Campos MM, Calixto JB
Biochemical pharmacology 2009 Apr 1;77(7):1223-35
Biochemical pharmacology 2009 Apr 1;77(7):1223-35
Mechanisms underlying the nociceptive responses induced by platelet-activating factor (PAF) in the rat paw.
Marotta DM, Costa R, Motta EM, Fernandes ES, Medeiros R, Quintão NL, Campos MM, Calixto JB
Biochemical pharmacology 2009 Apr 1;77(7):1223-35
Biochemical pharmacology 2009 Apr 1;77(7):1223-35
Tumor necrosis factor alpha: activity dependent expression and promotion of cortical column sleep in rats.
Churchill L, Rector DM, Yasuda K, Fix C, Rojas MJ, Yasuda T, Krueger JM
Neuroscience 2008 Sep 22;156(1):71-80
Neuroscience 2008 Sep 22;156(1):71-80
Supraspinal glial-neuronal interactions contribute to descending pain facilitation.
Wei F, Guo W, Zou S, Ren K, Dubner R
The Journal of neuroscience : the official journal of the Society for Neuroscience 2008 Oct 15;28(42):10482-95
The Journal of neuroscience : the official journal of the Society for Neuroscience 2008 Oct 15;28(42):10482-95
Non-steroidal anti-inflammatory drug-induced small intestinal damage is Toll-like receptor 4 dependent.
Watanabe T, Higuchi K, Kobata A, Nishio H, Tanigawa T, Shiba M, Tominaga K, Fujiwara Y, Oshitani N, Asahara T, Nomoto K, Takeuchi K, Arakawa T
Gut 2008 Feb;57(2):181-7
Gut 2008 Feb;57(2):181-7
Deleterious role of TNF-alpha in retinal ischemia-reperfusion injury.
Berger S, Savitz SI, Nijhawan S, Singh M, David J, Rosenbaum PS, Rosenbaum DM
Investigative ophthalmology & visual science 2008 Aug;49(8):3605-10
Investigative ophthalmology & visual science 2008 Aug;49(8):3605-10
Wnt-induced secreted protein-1 is a prohypertrophic and profibrotic growth factor.
Colston JT, de la Rosa SD, Koehler M, Gonzales K, Mestril R, Freeman GL, Bailey SR, Chandrasekar B
American journal of physiology. Heart and circulatory physiology 2007 Sep;293(3):H1839-46
American journal of physiology. Heart and circulatory physiology 2007 Sep;293(3):H1839-46
TNFalpha and TNF receptor 1 expression in the mixed neuronal-glial cultures of hippocampal dentate gyrus exposed to glutamate or trimethyltin.
Figiel I, Dzwonek K
Brain research 2007 Feb 2;1131(1):17-28
Brain research 2007 Feb 2;1131(1):17-28
Thrombin induces neurodegeneration and microglial activation in the cortex in vivo and in vitro: proteolytic and non-proteolytic actions.
Lee DY, Park KW, Jin BK
Biochemical and biophysical research communications 2006 Aug 4;346(3):727-38
Biochemical and biophysical research communications 2006 Aug 4;346(3):727-38
TNFalpha-induced AMPA-receptor trafficking in CNS neurons; relevance to excitotoxicity?
Leonoudakis D, Braithwaite SP, Beattie MS, Beattie EC
Neuron glia biology 2004 Aug;1(3):263-73
Neuron glia biology 2004 Aug;1(3):263-73
Differential cytokine response in interstitial fluid in skin and serum during experimental inflammation in rats.
Nedrebø T, Reed RK, Jonsson R, Berg A, Wiig H
The Journal of physiology 2004 Apr 1;556(Pt 1):193-202
The Journal of physiology 2004 Apr 1;556(Pt 1):193-202
Glucocorticoids play a fundamental role in protecting the brain during innate immune response.
Nadeau S, Rivest S
The Journal of neuroscience : the official journal of the Society for Neuroscience 2003 Jul 2;23(13):5536-44
The Journal of neuroscience : the official journal of the Society for Neuroscience 2003 Jul 2;23(13):5536-44
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- TNF-alpha in Rat Splenocytes. TNF-alpha was detected in immersion fixed rat splenocytes using Goat Anti-Rat TNF-alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-510-NA) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasmic. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cytotoxicity Induced by TNF-alpha and Neutralization by Rat TNF-alpha Antibody. Recombinant Rat TNF-alpha (Catalog # 510-RT) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin. Cytotoxicity elicited by Recombinant Rat TNF-alpha (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat TNF-alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-510-NA). The ND50 is typically 0.15-0.75 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).