Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AF690 - Provider product page
- Provider
- R&D Systems
- Product name
- Porcine TNF-alpha Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects porcine TNF-alpha in direct ELISAs and Western blots.
- Reactivity
- Porcine
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P23563
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Atorvastatin treatment improves survival and effects of implanted mesenchymal stem cells in post-infarct swine hearts.
Stent implantation in coronary porcine arteries is associated with early activation of TNFalpha and TNFalpha receptor II expression.
Macrophage transcriptional responses following in vitro infection with a highly virulent African swine fever virus isolate.
Liposomal delivery of heat shock protein 72 into renal tubular cells blocks nuclear factor-kappaB activation, tumor necrosis factor-alpha production, and subsequent ischemia-induced apoptosis.
Liposomal delivery of heat shock protein 72 into renal tubular cells blocks nuclear factor-kappaB activation, tumor necrosis factor-alpha production, and subsequent ischemia-induced apoptosis.
Yang YJ, Qian HY, Huang J, Geng YJ, Gao RL, Dou KF, Yang GS, Li JJ, Shen R, He ZX, Lu MJ, Zhao SH
European heart journal 2008 Jun;29(12):1578-90
European heart journal 2008 Jun;29(12):1578-90
Stent implantation in coronary porcine arteries is associated with early activation of TNFalpha and TNFalpha receptor II expression.
Hadjadj S, Cloutier I, Geoffroy P, Tanguay JF
Atherosclerosis 2007 May;192(1):25-32
Atherosclerosis 2007 May;192(1):25-32
Macrophage transcriptional responses following in vitro infection with a highly virulent African swine fever virus isolate.
Zhang F, Hopwood P, Abrams CC, Downing A, Murray F, Talbot R, Archibald A, Lowden S, Dixon LK
Journal of virology 2006 Nov;80(21):10514-21
Journal of virology 2006 Nov;80(21):10514-21
Liposomal delivery of heat shock protein 72 into renal tubular cells blocks nuclear factor-kappaB activation, tumor necrosis factor-alpha production, and subsequent ischemia-induced apoptosis.
Meldrum KK, Burnett AL, Meng X, Misseri R, Shaw MB, Gearhart JP, Meldrum DR
Circulation research 2003 Feb 21;92(3):293-9
Circulation research 2003 Feb 21;92(3):293-9
Liposomal delivery of heat shock protein 72 into renal tubular cells blocks nuclear factor-kappaB activation, tumor necrosis factor-alpha production, and subsequent ischemia-induced apoptosis.
Meldrum KK, Burnett AL, Meng X, Misseri R, Shaw MB, Gearhart JP, Meldrum DR
Circulation research 2003 Feb 21;92(3):293-9
Circulation research 2003 Feb 21;92(3):293-9
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- TNF-alpha in Porcine PBMCs. TNF-alpha was detected in immersion fixed porcine peripheral blood mononuclear cells (PBMCs) treated with calcium ionomycin and PMA using Goat Anti-Porcine TNF-alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF690) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cytotoxicity Induced by TNF-alpha and Neutralization by Porcine TNF-alpha Antibody. Recombinant Porcine TNF-alpha (Catalog # 690-PT) induces cytotoxicity in the the PK-15 porcine kidney epithelial cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Cytotoxicity elicited by Recombinant Porcine TNF-alpha (0.05 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Porcine TNF-alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF690). The ND50 is typically 0.2-0.8 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).