H00007124-M02

antibody from Abnova Corporation

Targeting: TNF DIF, TNF-alpha, TNFA, TNFSF2

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00007124-M02

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00007124-M02, RRID:AB_437069

Product name

TNF monoclonal antibody (M02), clone 1C3-A1-F4

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant TNF.

Antigen sequence

MSTESMIRDVELAEEALPKKTGGPQGSRRCLFLSL
FSFLIVAGATTLFCLLHFGVIGPQREEFPRDLSLI
SPLAQAVRSSSRTPSDKPVAHVVANPQAEGQLQWL
NRRANALLANGVELRDNQLVVPSEGLYLIYSQVLF
KGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKS
PCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSA
EINRPDYLDFAESGQVYFGIIAL


Isotype

IgG

Antibody clone number

1C3-A1-F4

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

TNF monoclonal antibody (M02), clone S9. Western Blot analysis of TNF expression in rat testis.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged TNF is approximately 0.03ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol