NBP2-52655-0.2mg

antibody from Novus Biologicals

Targeting: TNF DIF, TNF-alpha, TNFA, TNFSF2

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

NBP2-52655-0.2mg

Provider

Novus Biologicals

Product name

Rabbit Monoclonal TNF-alpha Antibody

Antibody type

Monoclonal

Description

Protein A purified. Binds to soluble and transmembrane forms of TNF-alpha and neutralizes its biological activity. Does not bind to TNF-beta.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

0.2 mg

Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: TNF-alpha Antibody (cA2 (Infliximab)) [NBP2-52655] - Western Blot using anti-TNF alpha antibody. Mouse liver (lane 1), mouse spinal cord (lane 2), mouse testis (lane 3), mouse colon (lane 4) and human thyroid tumour (lane 5) samples were resolved on a 10% SDS PAGE gel and blots probed at 1.5 ug/ml before being detected by a secondary antibody. The expected band size (269 kDa) is seen in the human-derived samples, as well as a band corresponding to dimers.