NBP2-52679-0.2mg

antibody from Novus Biologicals

Targeting: TNF DIF, TNF-alpha, TNFA, TNFSF2

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Data presented on provider website)

Antibody Data

Product number

NBP2-52679-0.2mg

Provider

Novus Biologicals

Product name

Rabbit Monoclonal TNF-alpha Antibody

Antibody type

Monoclonal

Description

Protein A purified. The antibody binds to human TNF-alpha with a Kd of 0.087 nM.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

0.2 mg

Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: TNF-alpha Antibody (CDP 571 (Humicade)) [NBP2-52679] - Western Blot using anti-TNFalpha antibody Rat liver (lane 1), rat spinal cord (lane 2), mouse testis (lane 3), rat colon (lane 4) and human thyroid tumour (lane 5) samples were resolved on a 10% SDS PAGE gel and blots probed at 1 ug/ml before being detected by a secondary antibody. The expected band size for TNFalpha is 26 kDa, however dimers and other higher order complexes are well described in the literature too (c.f. Petersen 1989, PMID: 2583226).

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: TNF-alpha Antibody (CDP 571 (Humicade)) [NBP2-52679] - Immunohistochemical analysis of rat liver using anti-TNFalpha antibody NBP2-52679 Formalin fixed rat liver slices were stained at 5 ug/ml.