Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [2]
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Validation data
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- Product number
- 10-4095 - Provider product page
- Provider
- ABEOMICS Inc.
- Product name
- Anti-Human TNF-alpha Antibody
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Full-length recombinant human TNF a
lpha was used as the immunogen for
this antibody.- Isotype
- IgG
- Antibody clone number
- ABM3D89
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeat freez thawing
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Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Intracellular flowcytometric analysis of TNF Alpha in HeLa cells using 2 µg/10^6 cells of Anti-TNF alpha (Clone: ABM3D89). Green represents isotype control and red represents Anti-TNF alpha (10-4095 Abeomics). Goat anti-mouse PE conjugated was used as secondary control.
- Protocol
- Protocol
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Intracellular flow cytometric analysis of TNF alpha on PMA and Ionomycin treated PBMCs using 2 µg/10^6 cells of TNF alpha (10-4095) antibody. Green represents isotype control , red represents anti-TNF alpha antibody (ABM3D89). Goat anti-mouse PE conjugate was used as secondary. The cells are lymphocyte gated.
- Protocol
- Protocol