Antibody data
- Antibody Data
- Antigen structure
- References [28]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- A00002-5 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-TNF alpha/Tnf Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Rabbit IgG polyclonal antibody for TNF alpha/Tnf detection. Tested with WB, ICC/IF, FCM, Direct ELISA in Mouse;Rat.
- Reactivity
- Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Taraxacum officinale-derived exosome-like nanovesicles modulate gut metabolites to prevent intermittent hypoxia-induced hypertension.
Adipose-Derived Stem Cell Exosomes Inhibit Hypertrophic Scaring Formation by Regulating Th17/Treg Cell Balance.
Treatment of ulcerative colitis with Wu-Mei-Wan by inhibiting intestinal inflammatory response and repairing damaged intestinal mucosa.
Key Role of Pro-inflammatory Cytokines in the Toxic Effect of Fluoride on Hepa1-6 Cells.
MMP-13 enzyme and pH responsive theranostic nanoplatform for osteoarthritis.
Adolescent cocaine exposure induces prolonged synaptic modifications in medial prefrontal cortex of adult rats.
Gypsophila elegans isoorientin-2″-O-α-l-arabinopyranosyl ameliorates porcine serum-induced immune liver fibrosis by inhibiting NF-κB signaling pathway and suppressing HSC activation.
Pentoxifylline inhibits pulmonary inflammation induced by infrarenal aorticcross-clamping dependent of adenosine receptor A2A.
Cerebralcare Granule(®), a Chinese Herb Compound Preparation, Attenuates D-Galactose Induced Memory Impairment in Mice.
Puerarin exerts the protective effect against chemical induced dysmetabolism in rats.
Resveratrol fails to affect cocaine conditioned place preference behavior, but alleviates anxiety-like behaviors in cocaine withdrawn rats.
Hydrogen sulfide accelerates wound healing in diabetic rats.
Effects of compound Ginkgo biloba on intestinal permeability in rats with alcohol-induced liver injury.
Protective effect of melatonin on cigarette smoke-induced restenosis in rat carotid arteries after balloon injury.
Long-term resveratrol treatment prevents ovariectomy-induced osteopenia in rats without hyperplastic effects on the uterus.
Protective effect of iridoid glycosides from Paederia scandens (LOUR.) MERRILL (Rubiaceae) on uric acid nephropathy rats induced by yeast and potassium oxonate.
Puerarin protects brain tissue against cerebral ischemia/reperfusion injury by inhibiting the inflammatory response.
Effects of selective hypothermia on blood-brain barrier integrity and tight junction protein expression levels after intracerebral hemorrhage in rats.
Alteration in P-glycoprotein at the blood-brain barrier in the early period of MCAO in rats.
Relationship between expression of COX-2, TNF-α, IL-6 and autoimmune-type recurrent miscarriage.
Protective roles of quercetin in acute myocardial ischemia and reperfusion injury in rats.
Protective effects of propofol on endotoxemia-induced acute kidney injury in rats.
Protective effects of lithium treatment for spatial memory deficits induced by tau hyperphosphorylation in splenectomized rats.
Effects of triptolide from Radix Tripterygium wilfordii (Leigongteng) on cartilage cytokines and transcription factor NF-kappaB: a study on induced arthritis in rats.
The inhibitory effects of cholalic acid and hyodeoxycholalic acid on the expression of TNFalpha and IL-1beta after cerebral ischemia in rats.
IL-17 stimulates migration of carotid artery vascular smooth muscle cells in an MMP-9 dependent manner via p38 MAPK and ERK1/2-dependent NF-kappaB and AP-1 activation.
Crocetin attenuates palmitate-induced insulin insensitivity and disordered tumor necrosis factor-alpha and adiponectin expression in rat adipocytes.
Effect of Huwentoxin-I on the Fas and TNF apoptosis pathway in the hippocampus of rat with global cerebral ischemia.
Zhang X, Pan Z, Wang Y, Liu P, Hu K
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2023 May;161:114572
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2023 May;161:114572
Adipose-Derived Stem Cell Exosomes Inhibit Hypertrophic Scaring Formation by Regulating Th17/Treg Cell Balance.
Wang H, Li Y, Yue Z, Liu Y, Chen Q, Hu D, Han J
BioMed research international 2022;2022:9899135
BioMed research international 2022;2022:9899135
Treatment of ulcerative colitis with Wu-Mei-Wan by inhibiting intestinal inflammatory response and repairing damaged intestinal mucosa.
Yan S, Wang P, Wei H, Jia R, Zhen M, Li Q, Xue C, Li J
Phytomedicine : international journal of phytotherapy and phytopharmacology 2022 Oct;105:154362
Phytomedicine : international journal of phytotherapy and phytopharmacology 2022 Oct;105:154362
Key Role of Pro-inflammatory Cytokines in the Toxic Effect of Fluoride on Hepa1-6 Cells.
Zhang Y, Zhou BH, Tan PP, Chen Y, Miao CY, Wang HW
Biological trace element research 2020 Sep;197(1):115-122
Biological trace element research 2020 Sep;197(1):115-122
MMP-13 enzyme and pH responsive theranostic nanoplatform for osteoarthritis.
Lan Q, Lu R, Chen H, Pang Y, Xiong F, Shen C, Qin Z, Zheng L, Xu G, Zhao J
Journal of nanobiotechnology 2020 Aug 27;18(1):117
Journal of nanobiotechnology 2020 Aug 27;18(1):117
Adolescent cocaine exposure induces prolonged synaptic modifications in medial prefrontal cortex of adult rats.
Zhu W, Ge X, Gao P, Li M, Guan Y, Guan X
Brain structure & function 2018 May;223(4):1829-1838
Brain structure & function 2018 May;223(4):1829-1838
Gypsophila elegans isoorientin-2″-O-α-l-arabinopyranosyl ameliorates porcine serum-induced immune liver fibrosis by inhibiting NF-κB signaling pathway and suppressing HSC activation.
Bai F, Huang Q, Wei J, Lv S, Chen Y, Liang C, Wei L, Lu Z, Lin X
International immunopharmacology 2018 Jan;54:60-67
International immunopharmacology 2018 Jan;54:60-67
Pentoxifylline inhibits pulmonary inflammation induced by infrarenal aorticcross-clamping dependent of adenosine receptor A2A.
Li H, Tan G, Tong L, Han P, Zhang F, Liu B, Sun X
American journal of translational research 2016;8(5):2210-21
American journal of translational research 2016;8(5):2210-21
Cerebralcare Granule(®), a Chinese Herb Compound Preparation, Attenuates D-Galactose Induced Memory Impairment in Mice.
Qu Z, Yang H, Zhang J, Huo L, Chen H, Li Y, Liu C, Gao W
Neurochemical research 2016 Sep;41(9):2199-214
Neurochemical research 2016 Sep;41(9):2199-214
Puerarin exerts the protective effect against chemical induced dysmetabolism in rats.
Li R, Song J, Wu W, Wu X, Su M
Gene 2016 Dec 31;595(2):168-174
Gene 2016 Dec 31;595(2):168-174
Resveratrol fails to affect cocaine conditioned place preference behavior, but alleviates anxiety-like behaviors in cocaine withdrawn rats.
Hu P, Zhu W, Zhu C, Jin L, Guan Y, Guan X
Psychopharmacology 2016 Apr;233(7):1279-87
Psychopharmacology 2016 Apr;233(7):1279-87
Hydrogen sulfide accelerates wound healing in diabetic rats.
Wang G, Li W, Chen Q, Jiang Y, Lu X, Zhao X
International journal of clinical and experimental pathology 2015;8(5):5097-104
International journal of clinical and experimental pathology 2015;8(5):5097-104
Effects of compound Ginkgo biloba on intestinal permeability in rats with alcohol-induced liver injury.
Li H, Qiu P, Wang J, Niu C, Pan S
Food & function 2015 Feb;6(2):470-8
Food & function 2015 Feb;6(2):470-8
Protective effect of melatonin on cigarette smoke-induced restenosis in rat carotid arteries after balloon injury.
Yang GH, Li YC, Wang ZQ, Liu B, Ye W, Ni L, Zeng R, Miao SY, Wang LF, Liu CW
Journal of pineal research 2014 Nov;57(4):451-8
Journal of pineal research 2014 Nov;57(4):451-8
Long-term resveratrol treatment prevents ovariectomy-induced osteopenia in rats without hyperplastic effects on the uterus.
Zhao H, Li X, Li N, Liu T, Liu J, Li Z, Xiao H, Li J
The British journal of nutrition 2014 Mar 14;111(5):836-46
The British journal of nutrition 2014 Mar 14;111(5):836-46
Protective effect of iridoid glycosides from Paederia scandens (LOUR.) MERRILL (Rubiaceae) on uric acid nephropathy rats induced by yeast and potassium oxonate.
Hou SX, Zhu WJ, Pang MQ, Jeffry J, Zhou LL
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2014 Feb;64:57-64
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2014 Feb;64:57-64
Puerarin protects brain tissue against cerebral ischemia/reperfusion injury by inhibiting the inflammatory response.
Zhou F, Wang L, Liu P, Hu W, Zhu X, Shen H, Yao Y
Neural regeneration research 2014 Dec 1;9(23):2074-80
Neural regeneration research 2014 Dec 1;9(23):2074-80
Effects of selective hypothermia on blood-brain barrier integrity and tight junction protein expression levels after intracerebral hemorrhage in rats.
Sun H, Tang Y, Guan X, Li L, Wang D
Biological chemistry 2013 Oct;394(10):1317-24
Biological chemistry 2013 Oct;394(10):1317-24
Alteration in P-glycoprotein at the blood-brain barrier in the early period of MCAO in rats.
Cen J, Liu L, Li MS, He L, Wang LJ, Liu YQ, Liu M, Ji BS
The Journal of pharmacy and pharmacology 2013 May;65(5):665-72
The Journal of pharmacy and pharmacology 2013 May;65(5):665-72
Relationship between expression of COX-2, TNF-α, IL-6 and autoimmune-type recurrent miscarriage.
Hua F, Li CH, Wang H, Xu HG
Asian Pacific journal of tropical medicine 2013 Dec;6(12):990-4
Asian Pacific journal of tropical medicine 2013 Dec;6(12):990-4
Protective roles of quercetin in acute myocardial ischemia and reperfusion injury in rats.
Jin HB, Yang YB, Song YL, Zhang YC, Li YR
Molecular biology reports 2012 Dec;39(12):11005-9
Molecular biology reports 2012 Dec;39(12):11005-9
Protective effects of propofol on endotoxemia-induced acute kidney injury in rats.
Cui WY, Tian AY, Bai T
Clinical and experimental pharmacology & physiology 2011 Nov;38(11):747-54
Clinical and experimental pharmacology & physiology 2011 Nov;38(11):747-54
Protective effects of lithium treatment for spatial memory deficits induced by tau hyperphosphorylation in splenectomized rats.
Tan WF, Cao XZ, Wang JK, Lv HW, Wu BY, Ma H
Clinical and experimental pharmacology & physiology 2010 Oct;37(10):1010-5
Clinical and experimental pharmacology & physiology 2010 Oct;37(10):1010-5
Effects of triptolide from Radix Tripterygium wilfordii (Leigongteng) on cartilage cytokines and transcription factor NF-kappaB: a study on induced arthritis in rats.
Xiao C, Zhou J, He Y, Jia H, Zhao L, Zhao N, Lu A
Chinese medicine 2009 Jul 2;4:13
Chinese medicine 2009 Jul 2;4:13
The inhibitory effects of cholalic acid and hyodeoxycholalic acid on the expression of TNFalpha and IL-1beta after cerebral ischemia in rats.
Hua Q, Zhu XL, Li PT, Liu Y, Zhang N, Xu Y, Jia X
Archives of pharmacal research 2009 Jan;32(1):65-73
Archives of pharmacal research 2009 Jan;32(1):65-73
IL-17 stimulates migration of carotid artery vascular smooth muscle cells in an MMP-9 dependent manner via p38 MAPK and ERK1/2-dependent NF-kappaB and AP-1 activation.
Cheng G, Wei L, Xiurong W, Xiangzhen L, Shiguang Z, Songbin F
Cellular and molecular neurobiology 2009 Dec;29(8):1161-8
Cellular and molecular neurobiology 2009 Dec;29(8):1161-8
Crocetin attenuates palmitate-induced insulin insensitivity and disordered tumor necrosis factor-alpha and adiponectin expression in rat adipocytes.
Xi L, Qian Z, Xu G, Zhou C, Sun S
British journal of pharmacology 2007 Jul;151(5):610-7
British journal of pharmacology 2007 Jul;151(5):610-7
Effect of Huwentoxin-I on the Fas and TNF apoptosis pathway in the hippocampus of rat with global cerebral ischemia.
Wang YR, Liu RY, Wang LC, Mao HF, Chen JQ
Toxicon : official journal of the International Society on Toxinology 2007 Dec 15;50(8):1085-94
Toxicon : official journal of the International Society on Toxinology 2007 Dec 15;50(8):1085-94
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of TNF Alpha/Tnf using anti-TNF Alpha/Tnf antibody (A00002-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF Alpha/Tnf antigen affinity purified polyclonal antibody (Catalog # A00002-5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNF Alpha/Tnf at approximately 25KD. The expected band size for TNF Alpha/Tnf is at 25KD.
- Additional image