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- Antibody Data
- Antigen structure
- References [22]
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- Product number
- M302B - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TNF alpha Monoclonal Antibody (2TNF-H33), Biotin
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- M302B targets TNF Alpha in ELISA, and WB applications and shows reactivity with Human samples. The M303 antibody (clone 2TNF-H34A) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody M302B (biotinylated conjugate of clone 2TNG-H33).
- Conjugate
- Biotin
- Antibody clone number
- 2TNF-H33
- Concentration
- 0.5 mg/mL
Submitted references Small tumor necrosis factor receptor biologics inhibit the tumor necrosis factor-p38 signalling axis and inflammation.
Reduced frequency of a CD14+ CD16+ monocyte subset with high Toll-like receptor 4 expression in cord blood compared to adult blood contributes to lipopolysaccharide hyporesponsiveness in newborns.
Anandamide suppresses proliferation and cytokine release from primary human T-lymphocytes mainly via CB2 receptors.
Cytokines across the night in chronic fatigue syndrome with and without fibromyalgia.
Efficient immobilization of a ligand antibody with high antigen-binding activity by use of a polystyrene-binding peptide and an intelligent microtiter plate.
Sesquiterpene lactones induce distinct forms of cell death that modulate human monocyte-derived macrophage responses.
Protein microarray platform for the multiplex analysis of biomarkers in human sera.
The immunostimulant RU41740 from Klebsiella pneumoniae activates human cells in whole blood to potentially stimulate innate and adaptive immune responses.
Thalidomide-based TNF-alpha inhibitors for neurodegenerative diseases.
Generation of three-dimensional pannus-like tissues in vitro from single cell suspensions of synovial fluid cells from arthritis patients.
Host factors impacting the innate response in humans to the candidate adjuvants RC529 and monophosphoryl lipid A.
Long-term treatment with etanercept significantly reduces the number of proinflammatory cytokine-secreting peripheral blood mononuclear cells in patients with rheumatoid arthritis.
Thiothalidomides: novel isosteric analogues of thalidomide with enhanced TNF-alpha inhibitory activity.
Enhanced monocyte Th1 cytokine production in HCV-infected cryoglobulinemic patients.
Enhanced monocyte Th1 cytokine production in HCV-infected cryoglobulinemic patients.
Interferon--gamma control of EBV-transformed B cells: a role for CD8+ T cells that poorly kill EBV-infected cells.
Enhanced interleukin-12 and CD40 ligand activities but reduced Staphylococcus aureus Cowan 1-induced responses suggest a generalized and progressively impaired type 1 cytokine pattern for human schistosomiasis.
Enhanced interleukin-12 and CD40 ligand activities but reduced Staphylococcus aureus Cowan 1-induced responses suggest a generalized and progressively impaired type 1 cytokine pattern for human schistosomiasis.
Human dendritic cells are activated by dengue virus infection: enhancement by gamma interferon and implications for disease pathogenesis.
Suppression of TNF-alpha production in human mononuclear cells by an adenosine kinase inhibitor.
Regulation of interleukin-12 by complement receptor 3 signaling.
Regulation of interleukin-12 by complement receptor 3 signaling.
Mukaro VR, Quach A, Gahan ME, Boog B, Huang ZH, Gao X, Haddad C, Mahalingam S, Hii CS, Ferrante A
Nature communications 2018 Apr 10;9(1):1365
Nature communications 2018 Apr 10;9(1):1365
Reduced frequency of a CD14+ CD16+ monocyte subset with high Toll-like receptor 4 expression in cord blood compared to adult blood contributes to lipopolysaccharide hyporesponsiveness in newborns.
Pedraza-Sánchez S, Hise AG, Ramachandra L, Arechavaleta-Velasco F, King CL
Clinical and vaccine immunology : CVI 2013 Jul;20(7):962-71
Clinical and vaccine immunology : CVI 2013 Jul;20(7):962-71
Anandamide suppresses proliferation and cytokine release from primary human T-lymphocytes mainly via CB2 receptors.
Cencioni MT, Chiurchiù V, Catanzaro G, Borsellino G, Bernardi G, Battistini L, Maccarrone M
PloS one 2010 Jan 14;5(1):e8688
PloS one 2010 Jan 14;5(1):e8688
Cytokines across the night in chronic fatigue syndrome with and without fibromyalgia.
Nakamura T, Schwander SK, Donnelly R, Ortega F, Togo F, Broderick G, Yamamoto Y, Cherniack NS, Rapoport D, Natelson BH
Clinical and vaccine immunology : CVI 2010 Apr;17(4):582-7
Clinical and vaccine immunology : CVI 2010 Apr;17(4):582-7
Efficient immobilization of a ligand antibody with high antigen-binding activity by use of a polystyrene-binding peptide and an intelligent microtiter plate.
Kumada Y, Hamasaki K, Shiritani Y, Ohse T, Kishimoto M
Journal of biotechnology 2009 Jun 15;142(2):135-41
Journal of biotechnology 2009 Jun 15;142(2):135-41
Sesquiterpene lactones induce distinct forms of cell death that modulate human monocyte-derived macrophage responses.
López-Antón N, Hermann C, Murillo R, Merfort I, Wanner G, Vollmar AM, Dirsch VM
Apoptosis : an international journal on programmed cell death 2007 Jan;12(1):141-53
Apoptosis : an international journal on programmed cell death 2007 Jan;12(1):141-53
Protein microarray platform for the multiplex analysis of biomarkers in human sera.
Urbanowska T, Mangialaio S, Zickler C, Cheevapruk S, Hasler P, Regenass S, Legay F
Journal of immunological methods 2006 Oct 20;316(1-2):1-7
Journal of immunological methods 2006 Oct 20;316(1-2):1-7
The immunostimulant RU41740 from Klebsiella pneumoniae activates human cells in whole blood to potentially stimulate innate and adaptive immune responses.
Pedraza-Sánchez S, González-Hernández Y, Escobar-Gutiérrez A, Ramachandra L
International immunopharmacology 2006 Apr;6(4):635-46
International immunopharmacology 2006 Apr;6(4):635-46
Thalidomide-based TNF-alpha inhibitors for neurodegenerative diseases.
Greig NH, Giordano T, Zhu X, Yu QS, Perry TA, Holloway HW, Brossi A, Rogers JT, Sambamurti K, Lahiri DK
Acta neurobiologiae experimentalis 2004;64(1):1-9
Acta neurobiologiae experimentalis 2004;64(1):1-9
Generation of three-dimensional pannus-like tissues in vitro from single cell suspensions of synovial fluid cells from arthritis patients.
Solomon S, Masilamani M, Mohanty S, Schwab JE, Boneberg EM, Illges H
Rheumatology international 2004 Mar;24(2):71-6
Rheumatology international 2004 Mar;24(2):71-6
Host factors impacting the innate response in humans to the candidate adjuvants RC529 and monophosphoryl lipid A.
Tiberio L, Fletcher L, Eldridge JH, Duncan DD
Vaccine 2004 Mar 29;22(11-12):1515-23
Vaccine 2004 Mar 29;22(11-12):1515-23
Long-term treatment with etanercept significantly reduces the number of proinflammatory cytokine-secreting peripheral blood mononuclear cells in patients with rheumatoid arthritis.
Schotte H, Schlüter B, Willeke P, Mickholz E, Schorat MA, Domschke W, Gaubitz M
Rheumatology (Oxford, England) 2004 Aug;43(8):960-4
Rheumatology (Oxford, England) 2004 Aug;43(8):960-4
Thiothalidomides: novel isosteric analogues of thalidomide with enhanced TNF-alpha inhibitory activity.
Zhu X, Giordano T, Yu QS, Holloway HW, Perry TA, Lahiri DK, Brossi A, Greig NH
Journal of medicinal chemistry 2003 Nov 20;46(24):5222-9
Journal of medicinal chemistry 2003 Nov 20;46(24):5222-9
Enhanced monocyte Th1 cytokine production in HCV-infected cryoglobulinemic patients.
Loffreda S, Muratori P, Muratori L, Mele L, Bianchi FB, Lenzi M
Journal of hepatology 2003 Feb;38(2):230-6
Journal of hepatology 2003 Feb;38(2):230-6
Enhanced monocyte Th1 cytokine production in HCV-infected cryoglobulinemic patients.
Loffreda S, Muratori P, Muratori L, Mele L, Bianchi FB, Lenzi M
Journal of hepatology 2003 Feb;38(2):230-6
Journal of hepatology 2003 Feb;38(2):230-6
Interferon--gamma control of EBV-transformed B cells: a role for CD8+ T cells that poorly kill EBV-infected cells.
Shi Y, Lutz CT
Viral immunology 2002;15(1):213-25
Viral immunology 2002;15(1):213-25
Enhanced interleukin-12 and CD40 ligand activities but reduced Staphylococcus aureus Cowan 1-induced responses suggest a generalized and progressively impaired type 1 cytokine pattern for human schistosomiasis.
Montenegro SM, Abath FG, Domingues AL, Melo WG, Morais CN, Coutinho EM, Mahanty S, Wynn TA
Infection and immunity 2002 Nov;70(11):5903-12
Infection and immunity 2002 Nov;70(11):5903-12
Enhanced interleukin-12 and CD40 ligand activities but reduced Staphylococcus aureus Cowan 1-induced responses suggest a generalized and progressively impaired type 1 cytokine pattern for human schistosomiasis.
Montenegro SM, Abath FG, Domingues AL, Melo WG, Morais CN, Coutinho EM, Mahanty S, Wynn TA
Infection and immunity 2002 Nov;70(11):5903-12
Infection and immunity 2002 Nov;70(11):5903-12
Human dendritic cells are activated by dengue virus infection: enhancement by gamma interferon and implications for disease pathogenesis.
Libraty DH, Pichyangkul S, Ajariyakhajorn C, Endy TP, Ennis FA
Journal of virology 2001 Apr;75(8):3501-8
Journal of virology 2001 Apr;75(8):3501-8
Suppression of TNF-alpha production in human mononuclear cells by an adenosine kinase inhibitor.
Eigler A, Matschke V, Hartmann G, Erhardt S, Boyle D, Firestein GS, Endres S
Journal of leukocyte biology 2000 Jul;68(1):97-103
Journal of leukocyte biology 2000 Jul;68(1):97-103
Regulation of interleukin-12 by complement receptor 3 signaling.
Marth T, Kelsall BL
The Journal of experimental medicine 1997 Jun 2;185(11):1987-95
The Journal of experimental medicine 1997 Jun 2;185(11):1987-95
Regulation of interleukin-12 by complement receptor 3 signaling.
Marth T, Kelsall BL
The Journal of experimental medicine 1997 Jun 2;185(11):1987-95
The Journal of experimental medicine 1997 Jun 2;185(11):1987-95
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Sandwich ELISA analysis of Human TNF alpha Biotinylated MAb (Product # M302B) was performed using components of Thermo Fisher Scientific Human TNF alpha Colorimetric ELISA Kit (Product # EH3TNFA) by loading 50 µL per well of Recombinant Human TNF alpha in octuplicate at 1000, 500, 250, 125, 62.5, 31.2, 15.6, and 0 pg/mL across a 5 µg/mL rabbit anti-Human TNF pre-coated plate and incubated for 1 hour at room temperature. The plate was washed then incubated with 100 µl per well of Human TNF alpha Biotinylated MAb (Product # M302B) in duplicate at 2, 1, 0.5, and 0.25 µg/mL for 1 hour at room temperature. The plate was washed and incubated with 100 µl per well of Ultra Streptavidin-HRP (Product # N504) in all test wells at 1:1, 500 for 30 minutes at room temperature. The plate was washed then incubated with 100 µL per well of TMB substrate for 30 minutes at room temperature in the dark. The plate was then stopped with 0.16M sulfuric acid and absorbance was read on a spectrophotometer at 450-550 nm.
- Conjugate
- Biotin
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Sandwich ELISA analysis of Human TNF alpha Biotinylated MAb (Product # M302B) was performed using components of Thermo Fisher Scientific Human TNF alpha Colorimetric ELISA Kit (Product # EH3TNFA) by loading 50 µL per well of Recombinant Human TNF alpha in octuplicate at 1000, 500, 250, 125, 62.5, 31.2, 15.6, and 0 pg/mL across a 5 µg/mL rabbit anti-Human TNF pre-coated plate and incubated for 1 hour at room temperature. The plate was washed then incubated with 100 µl per well of Human TNF alpha Biotinylated MAb (Product # M302B) in duplicate at 2, 1, 0.5, and 0.25 µg/mL for 1 hour at room temperature. The plate was washed and incubated with 100 µl per well of Ultra Streptavidin-HRP (Product # N504) in all test wells at 1:1, 500 for 30 minutes at room temperature. The plate was washed then incubated with 100 µL per well of TMB substrate for 30 minutes at room temperature in the dark. The plate was then stopped with 0.16M sulfuric acid and absorbance was read on a spectrophotometer at 450-550 nm.
- Conjugate
- Biotin
