R1136P

antibody from Acris Antibodies GmbH

Targeting: TNF DIF, TNF-alpha, TNFA, TNFSF2

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Functional assay (Recommended by provider)

Antibody Data

Product number

R1136P

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#R1136P, RRID:AB_1008139

Product name

anti Tumor necrosis factor (TNF-alpha)

Antibody type

Polyclonal

Antigen

Repeated immunizations with Recombinant Human TNF alpha produced in E.coli.

Reactivity

Human

Host

Rabbit

Vial size

1 mg

Concentration

1.0 mg/ml (by UV Absorbance at 280 nm)

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Figure 3. Western Blot using TNF-alpha antibody. Membrane blocked in 1% BSA-TBS-T 30 min RT, Rabbit anti-TNF alpha added at 1/1000 in 1% BSA-TBS-T o/n 4°C, DyLight 649 Gaot anti-Rabbit added at 1/20,000 in MB-070 30 min RT.

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Figure 1. R1136P TNFa antibody staining of Formalin/PFA-Fixed Paraffin-Embedded sections of Human artery tissue sections. Sections were fixed in formaldehyde and subjected to heat mediated antigen retrieval in citrate buffer (pH 6.0). Slides were blocked for ten minutes with 1.5% serum. Primary antibody was diluted 1/100 and incubated with samples for 24 hours at 4°C. HRP-conjugated Goat anti-Rabbit antibody was used as the secondary antibody.

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Figure 2. Fluorescent Immunohistochemical staining of Formalin/PFA-Fixed Paraffin-Embedded Human colon sections by R1136P TNF alpha antibody. Sections were formaldehyde-fixed, then blocked in 10% serum for 2 hours at 20°C. The primary antibody was diluted 1/100 and incubated with the sample for 2 hours at 20°C. Alexa Fluor® 680 Goat polyclonal secondary antibody was used diluted 1/5000.