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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [11]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- IC210P - Provider product page
- Provider
- R&D Systems
- Product name
- Human TNF-alpha PE-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects cytoplasmic forms of human TNF-alpha .
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 6402
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references Serum microRNA181a: Correlates with the intracellular cytokine levels and a potential biomarker for acute graft-versus-host disease.
Expansion of anti-mesothelin specific CD4+ and CD8+ T cell responses in patients with pancreatic carcinoma.
NK cells from HCV-infected patients effectively induce apoptosis of activated primary human hepatic stellate cells in a TRAIL-, FasL- and NKG2D-dependent manner.
Acetaldehyde stimulates monocyte adhesion in a P-selectin- and TNFalpha-dependent manner.
Human CD4(+) T cells recognize an epitope within alpha-fetoprotein sequence and develop into TGF-beta-producing CD4(+) T cells.
Human CD4(+) T cells recognize an epitope within alpha-fetoprotein sequence and develop into TGF-beta-producing CD4(+) T cells.
Interaction between transmembrane TNF and TNFR1/2 mediates the activation of monocytes by contact with T cells.
Interaction between transmembrane TNF and TNFR1/2 mediates the activation of monocytes by contact with T cells.
Analysis of CD4+ T-Cell responses to a novel alpha-fetoprotein-derived epitope in hepatocellular carcinoma patients.
Different domains of Pseudomonas aeruginosa exoenzyme S activate distinct TLRs.
Different domains of Pseudomonas aeruginosa exoenzyme S activate distinct TLRs.
Xie L, Zhou F, Liu X, Fang Y, Yu Z, Song N, Kong F
Cytokine 2016 Sep;85:37-44
Cytokine 2016 Sep;85:37-44
Expansion of anti-mesothelin specific CD4+ and CD8+ T cell responses in patients with pancreatic carcinoma.
Chen Y, Ayaru L, Mathew S, Morris E, Pereira SP, Behboudi S
PloS one 2014;9(2):e88133
PloS one 2014;9(2):e88133
NK cells from HCV-infected patients effectively induce apoptosis of activated primary human hepatic stellate cells in a TRAIL-, FasL- and NKG2D-dependent manner.
Glässner A, Eisenhardt M, Krämer B, Körner C, Coenen M, Sauerbruch T, Spengler U, Nattermann J
Laboratory investigation; a journal of technical methods and pathology 2012 Jul;92(7):967-77
Laboratory investigation; a journal of technical methods and pathology 2012 Jul;92(7):967-77
Acetaldehyde stimulates monocyte adhesion in a P-selectin- and TNFalpha-dependent manner.
Redmond EM, Morrow D, Kundimi S, Miller-Graziano CL, Cullen JP
Atherosclerosis 2009 Jun;204(2):372-80
Atherosclerosis 2009 Jun;204(2):372-80
Human CD4(+) T cells recognize an epitope within alpha-fetoprotein sequence and develop into TGF-beta-producing CD4(+) T cells.
Alisa A, Boswell S, Pathan AA, Ayaru L, Williams R, Behboudi S
Journal of immunology (Baltimore, Md. : 1950) 2008 Apr 1;180(7):5109-17
Journal of immunology (Baltimore, Md. : 1950) 2008 Apr 1;180(7):5109-17
Human CD4(+) T cells recognize an epitope within alpha-fetoprotein sequence and develop into TGF-beta-producing CD4(+) T cells.
Alisa A, Boswell S, Pathan AA, Ayaru L, Williams R, Behboudi S
Journal of immunology (Baltimore, Md. : 1950) 2008 Apr 1;180(7):5109-17
Journal of immunology (Baltimore, Md. : 1950) 2008 Apr 1;180(7):5109-17
Interaction between transmembrane TNF and TNFR1/2 mediates the activation of monocytes by contact with T cells.
Rossol M, Meusch U, Pierer M, Kaltenhäuser S, Häntzschel H, Hauschildt S, Wagner U
Journal of immunology (Baltimore, Md. : 1950) 2007 Sep 15;179(6):4239-48
Journal of immunology (Baltimore, Md. : 1950) 2007 Sep 15;179(6):4239-48
Interaction between transmembrane TNF and TNFR1/2 mediates the activation of monocytes by contact with T cells.
Rossol M, Meusch U, Pierer M, Kaltenhäuser S, Häntzschel H, Hauschildt S, Wagner U
Journal of immunology (Baltimore, Md. : 1950) 2007 Sep 15;179(6):4239-48
Journal of immunology (Baltimore, Md. : 1950) 2007 Sep 15;179(6):4239-48
Analysis of CD4+ T-Cell responses to a novel alpha-fetoprotein-derived epitope in hepatocellular carcinoma patients.
Alisa A, Ives A, Pathan AA, Navarrete CV, Williams R, Bertoletti A, Behboudi S
Clinical cancer research : an official journal of the American Association for Cancer Research 2005 Sep 15;11(18):6686-94
Clinical cancer research : an official journal of the American Association for Cancer Research 2005 Sep 15;11(18):6686-94
Different domains of Pseudomonas aeruginosa exoenzyme S activate distinct TLRs.
Epelman S, Stack D, Bell C, Wong E, Neely GG, Krutzik S, Miyake K, Kubes P, Zbytnuik LD, Ma LL, Xie X, Woods DE, Mody CH
Journal of immunology (Baltimore, Md. : 1950) 2004 Aug 1;173(3):2031-40
Journal of immunology (Baltimore, Md. : 1950) 2004 Aug 1;173(3):2031-40
Different domains of Pseudomonas aeruginosa exoenzyme S activate distinct TLRs.
Epelman S, Stack D, Bell C, Wong E, Neely GG, Krutzik S, Miyake K, Kubes P, Zbytnuik LD, Ma LL, Xie X, Woods DE, Mody CH
Journal of immunology (Baltimore, Md. : 1950) 2004 Aug 1;173(3):2031-40
Journal of immunology (Baltimore, Md. : 1950) 2004 Aug 1;173(3):2031-40
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of TNF-alpha in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with PMA and Ca2+ ionomycin were stained with Mouse Anti-Human TNF-alpha PE-conjugated Monoclonal Antibody (Catalog # IC210P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
