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LearnPA5-96219
antibody from Invitrogen Antibodies
Targeting: EDA
ED1, ED1-A1, ED1-A2, EDA-A1, EDA-A2, EDA1, EDA2, HED, ODT1, XHED, XLHED
Western blot
ELISA
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [3]
- Immunohistochemistry [5]
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- Product number
- PA5-96219 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EDA Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Positive Samples: A-549, HepG2, BxPC-3, Mouse liver; Cellular Location: Cell membrane, Single-pass type II membrane protein Immunogen sequence: LELRSELRRE RGAESRLGGS GTPGTSGTLS SLGGLDPDSP ITSHLGQPSP KQQPLEPGEA ALHSDSQDGH QMALLNFFFP DEKPYSEEES RRVRRNKRSK SNEGADGPVK NKKKGKKAGP P
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.43 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of EDA in C6 cells. Samples were incubated with EDA Polyclonal antibody (Product # PA5-96219) using a dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of EDA in HepG2 cells. Samples were incubated with EDA Polyclonal antibody (Product # PA5-96219) using a dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of EDA in U-251MG cells. Samples were incubated with EDA Polyclonal antibody (Product # PA5-96219) using a dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of EDA in paraffin-embedded human uterine cancer. Samples were incubated with EDA Polyclonal antibody (Product # PA5-96219) using a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of EDA in paraffin-embedded rat liver. Samples were incubated with EDA Polyclonal antibody (Product # PA5-96219) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of EDA in paraffin-embedded mouse heart. Samples were incubated with EDA Polyclonal antibody (Product # PA5-96219) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of EDA in paraffin-embedded rat liver. Samples were incubated with EDA Polyclonal antibody (Product # PA5-96219) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of EDA in paraffin-embedded mouse heart. Samples were incubated with EDA Polyclonal antibody (Product # PA5-96219) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
