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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- PA5-53585 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MLSTD2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: YYEGKNVLLT GATGFLGKVL LEKLLRSCPK VNSVYVLVRQ KAGQTPQERV EEVLSGKLFD RLRDENPDFR EKIIAINSEL TQPKLALSEE DKEVIIDSTN IIFHCAATVR FNENLRDAVQ LNVIATRQLI LLAQQMKNLE VFM Highest antigen sequence identity to the following orthologs: Mouse - 96%, Rat - 97%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.10 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Peroxisomal-derived ether phospholipids link nucleotides to respirasome assembly.
Bennett CF, O'Malley KE, Perry EA, Balsa E, Latorre-Muro P, Riley CL, Luo C, Jedrychowski M, Gygi SP, Puigserver P
Nature chemical biology 2021 Jun;17(6):703-710
Nature chemical biology 2021 Jun;17(6):703-710
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of MLSTD2 in human cell line RT4 using a MLSTD2 Polyclonal Antibody (Product # PA5-53585) shows localization to peroxisomes.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Extended Data Fig. 6 Ether lipid synthesis is required for respirasome assembly from DHODH inhibition a , Western blot of FAR1 from cells treated with Brequinar in the presence or absence of uridine (n=2 experiments). b , Western blot of FAR1 from sgFAR1 cells (n=2 experiments). c , Lipidomic analysis of PC and PE subclass abundances in mitochondrial fractions isolated from sgFAR1 cells treated with Brequinar (min-max box plot, line at mean, n=3 biologically independent samples, Student's t-test with a two-stage linear step-up procedure of Benjamini, Krieger and Yekutieli, with Q = 5%, * left to right sgControl DMSO [compared to sgControl Breq] q= 0.0003, 0.0034, 0.0007, sgControl DMSO [compared to sgFAR1 DMSO] q =0.0208,
