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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- MAB5916 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Latrophilin 3/LPHN3 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Latrophilin 3/LPHN3 in direct ELISAs and human, mouse, and rat Latrophilin 3/LPHN3 in Western blots.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human and Rat Latrophilin 3/LPHN3 by Simple WesternTM. Simple Western lane view shows lysates of human brain (hypothalamus) tissue and rat brain tissue, loaded at 0.5 mg/mL. A specific band was detected for Latrophilin 3/LPHN3 at approximately 109 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse/Rat Latrophilin 3/LPHN3 Monoclonal Antibody (Catalog # MAB5916). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human, Mouse, and Rat Latrophilin 3/LPHN3 by Western Blot. Western blot shows lysates of human brain (hypothalamus) tissue, mouse brain (cerebellum) tissue, and rat brain tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Latrophilin 3/LPHN3 Monoclonal Antibody (Catalog # MAB5916) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Latrophilin 3/LPHN3 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
