Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF8277 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Pref-1/DLK1/FA1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse Pref-1/DLK1/FA1 in direct ELISAs and Western blots. In direct ELISAs, approximately 75% cross-reactivity with recombinant human Pref-1 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q09163- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse Pref-1/DLK1/FA1 by Western Blot. Western blot shows lysates of 3T3-L1 mouse embryonic fibroblast adipose-like cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Pref-1/DLK1/FA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8277) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Pref-1/DLK1/FA1 at approximately 45-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Pref-1/DLK1/FA1 in Mouse Embryo. Pref-1/DLK1/FA1 was detected in immersion fixed frozen sections of mouse embryonic lung using Goat Anti-Mouse Pref-1/DLK1/FA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8277) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Pref-1/DLK1/FA1 in 3T3-L1 Mouse Cell Line by Flow Cytometry. 3T3-L1 mouse embryonic fibroblast adipose-like cell line was stained with Goat Anti-Mouse Pref-1/DLK1/FA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8277, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
