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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [1]
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- Product number
- F52806 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Dlk1 Antibody
- Antibody type
- Polyclonal
- Antigen
- This Dlk1 antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 372-405 amino acids from the C-terminal region of mouse Dlk1.
- Description
- Antigen affinity purified antibody
- Reactivity
- Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 80 µl, 400 µl
- Storage
- Aliquot the Dlk1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of lysate from 1) mouse kidney, 2) mouse thymus and 3) rat lung tissue lysate using Dlk1 antibody. Observed molecular weight 41~60 kDa depending on glycosylation level.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HepG2 cells using Dlk1 antibody (green) compared to an isotype control of mouse IgG2b (blue); Ab was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-mouse lgG was used as the secondary Ab.
